2016
DOI: 10.1016/j.bpj.2015.11.1410
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In Cell Footprinting Coupled with Mass Spectrometry for the Structural Characterization of a Membrane Protein

Abstract: Membrane proteins are assembled through balanced interactions among protein, lipids and water. Studying their folding while maintaining the native lipid environment has been a necessary but challenging. Here we present a set of methods for analyzing key elements in membrane protein folding, including thermodynamic stability, compactness of the unfolded state and unfolding cooperativity under native conditions. The methods are based on steric trapping which couples unfolding of a doublybiotinylated protein to b… Show more

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