In-channel simplified decoupler with renewable electrochemical detection for microchip capillary electrophoresis

Recent developments in instrumentation for capillary electrophoresis and microchip‐capillary electrophoresis

“…(Lawrence, KS) [109], or ESA Biosciences (Chelmsford, MA) have been used in conjunction with CE. In order to minimize the effect of the separation potential the three main approaches [96, 110] have been proposed: placing the detection electrode aligned just outside the separation channel (end-channel) [111, 112], placing the detection electrode in the separation channel (in-channel, requires using an isolated potentiostat) [113], and using a decoupler to ground the separation voltage before it reaches the detector, typically made with Pd (off-channel) [114, 115]. As relevant examples of the latter approach, Scott Martin’s group has investigated several designs of decouplers and found that the optimal distance between the first electrode and the decoupler was 250 μm, while the distance between the two electrodes has been optimized at 100 μm [116, 117].…”

Recent developments in electrochemical flow detections—A review

“…Another innovation reported recently includes a simple application in the CE chip of a renewable electrode, which was reported for determination of dopamine and catechol with a Cu wire working electrode [258]. Significant improvement of the amperometric signal magnitude with application as a working electrode and as decoupler electrodes a nanoelectrode ensemble in comparison with a bulk electrode was also described [259].…”

Recent Developments in Polymer Microfluidic Devices with Capillary Electrophoresis and Electrochemical Detection

“…Glucose in human plasma was successfully determined with a linear response from 10 M to 1 mM. Recently, Lin et al used Pt decoupler and a renewable working electrode (Cu) for the electrochemical detection of dopamine and catechol analytes over the concentration range 10-1000 M [92]. In the CE chip, the Pt and Cu wires were aligned horizontally on the separation channel.…”