In-column “click” preparation of hydrophobic organic monolithic stationary phases for protein separation

Sun, Xiangli; He, Xiwen; Chen, Langxing; Zhang, Yukui

doi:10.1007/s00216-010-4390-4

Cited by 26 publications

(26 citation statements)

References 31 publications

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“…Then, lauryl methacrylate monomers were clicked onto the monoliths using either heat or UV initiation. Thiol-yne and CuAAC reactions were also utilized to achieve reactive porous polymer monoliths [8,124]. Zhang and coworkers produced poly(glycidyl methacrylate-codivinylbenzene), poly(GMA-co-DVB), and poly(vinylbenzyl chloride-codivinylbenzene), (poly(VBC-co-DVB), monoliths to provide the reactive sites for click chemistry [29].…”

Section: Polymeric Monolithic Columnsmentioning

confidence: 99%

Post-polymerization Modification of Surface-Bound Polymers

Théato

2015

Controlled Radical Polymerization at and From Solid Surfaces

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Surfaces that have been intricately functionalized with reactive polymers have attracted scientific attention recently because of their potential use in a broad range of applications. Polymers containing chemically reactive functional groups can be utilized for subsequent modification of various surfaces. Reactive polymeric surfaces can be produced by surface-initiated polymerization, such as atom transfer radical polymerization, nitroxide-mediated polymerization, and ring-opening metathesis polymerization. Such surfaces can subsequently undergo postpolymerization modification to alter their physicochemical properties. Postpolymerization modification has a number of advantages, including the fact that diverse polymer structures are rapidly accessible without individual synthesis; polymerization of new functional monomers can produce a variety of surfaces and interfaces; and other materials can be easily modified, which would be difficult using conventional direct polymerization. In addition, the libraries of chemical reactions and materials that can be used in post-polymerization modifications are abundant. Therefore, post-polymerization modification opens up new platforms for the facile and versatile modification of various surfaces. This chapter focuses on a discussion of post-polymerization modification of various surface-bound polymers, from planar surfaces to three-dimensional objects, and on the extended applications of the reactive surfaces.

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Section: Polymeric Monolithic Columnsmentioning

confidence: 99%

Post-polymerization Modification of Surface-Bound Polymers

Théato

2015

Controlled Radical Polymerization at and From Solid Surfaces

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“…Click chemistry, the term coined by Sharpless et al [101] has been heavily utilized by organic chemists for many years as a versatile toolbox of synthetic strategies, many of which have been used in separation science, for the modification of monolithic and other types of separation media [102,103,104,105,106,107,108,109,110]. The mechanistic aspects of the chemistries involved in these reactions are beyond the scope of this article and shall not be considered in detail here.…”

Section: Methacrylate Polymer Monolithsmentioning

confidence: 99%

Methacrylate Polymer Monoliths for Separation Applications

Groarke

Brabazon

2016

Materials

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This review summarizes the development of methacrylate-based polymer monoliths for separation science applications. An introduction to monoliths is presented, followed by the preparation methods and characteristics specific to methacrylate monoliths. Both traditional chemical based syntheses and emerging additive manufacturing methods are presented along with an analysis of the different types of functional groups, which have been utilized with methacrylate monoliths. The role of methacrylate based porous materials in separation science in industrially important chemical and biological separations are discussed, with particular attention given to the most recent developments and challenges associated with these materials. While these monoliths have been shown to be useful for a wide variety of applications, there is still scope for exerting better control over the porous architectures and chemistries obtained from the different fabrication routes. Conclusions regarding this previous work are drawn and an outlook towards future challenges and potential developments in this vibrant research area are presented. Discussed in particular are the potential of additive manufacturing for the preparation of monolithic structures with pre-defined multi-scale porous morphologies and for the optimization of surface reactive chemistries.

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“…In recent years, several studies have been performed on the preparation of monolithic supports via click-chemistry [22][23][24][25]. In-column preparation of a brush-type chiral stationary phase using click-chemistry and a silica monolith was performed [22].…”

Section: Introductionmentioning

confidence: 99%

“…The monoliths developed were used for protein separation [23]. In-column "click" preparation of hydrophobic organic monolithic stationary phases for protein separation was performed [24]. Click-chemistry was also used for the hydrophobisation of silica-based monoliths [25].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a monolithic, micro-immobilised enzyme reactor via click-chemistry

2012

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An immobilised enzyme reactor (IMER) in the form of capillary monolith was developed for a micro-liquid chromatography system. The plain monolith was obtained by in situ thermal copolymerisation of glycidyl methacrylate and ethylene dimethacrylate in a fused silica capillary (200 × 0.53 mm ID) by using n-propanol/1,4-butanediol as porogen. The enzyme, α-chymotrypsin (CT), was covalently attached onto the monolith via triazole ring formation by click-chemistry. For this purpose, the monolithic support was treated with sodium azide and reacted with the alkyne carrying enzyme derivative. CT was covalently linked to the monolith by triazole-ring formation. The activity behaviour of monolithic IMER was investigated in a micro-liquid chromatography system by using benzoyl-L-tyrosine ethyl ester (BTEE) as synthetic substrate. The effects of mobile-phase flow rate and substrate feed concentration on the final BTEE conversion were investigated under steady-state conditions. In the case of monolithic IMER, the final substrate conversion increased with increasing feed flow rate and increasing substrate feed concentration. Unusual behaviour was explained by the presence of convective diffusion in the macropores of monolith. The results indicated that the monolithic-capillary IMER proposed for micro-liquid chromatography had significant advantages with respect to particle-based conventional high-performance liquid chromatography-IMERs.

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In-column “click” preparation of hydrophobic organic monolithic stationary phases for protein separation

Cited by 26 publications

References 31 publications

Post-polymerization Modification of Surface-Bound Polymers

Post-polymerization Modification of Surface-Bound Polymers

Methacrylate Polymer Monoliths for Separation Applications

Synthesis of a monolithic, micro-immobilised enzyme reactor via click-chemistry

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