The push for decarbonization of internal combustion engines (ICEs) has spurred interest in alternative fuels, such as hydrogen and ammonia. To optimize combustion efficiency and reduce emissions, a closer look at the intake system and in-cylinder flows is crucial, especially when a hard-to-burn fuel, such as ammonia is utilized. In port fuel injection ICEs, airflow within cylinders profoundly affects combustion and emissions by influencing the air–fuel mixing phenomenon. Adjusting intake port openings is an important factor in controlling the in-cylinder airflow. In previous experiments with a transparent cylinder, tangential and helical ports demonstrated that varying the helical port’s opening significantly impacts flow velocities, swirl ratios, and swirl center positions (SCPs). In this study, we used a particle image velocimetry technique to investigate how the tangential port’s opening affects intake and in-cylinder flows. Flow velocities were assessed at different planes near the cylinder head, evaluating streamline maps, turbulent kinetic energy (TKE), and SCPs. Under the given experimental conditions, swirl flows were successfully generated early in the compression stroke when the tangential port opening exceeded 25%. Our findings emphasize the importance of minimizing TKE and SCP variation for successful swirl flow generation in engine cylinders equipped with both tangential and helical ports.