In depth investigation of the retention behavior of structurally related β-blockers on RP-HPLC column: Quality by design and quantitative structure-property relationship complementary approaches for optimization and validation

Hakiem, Ahmed Faried Abdel; Hamdy, Ahmed; Ali, Hassan Refat H.; Gomaa, Mohamed; Aboraia, Ahmed S.

doi:10.1016/j.jchromb.2021.122549

Cited by 7 publications

(4 citation statements)

References 39 publications

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“…The QbD is considered a prevalidation approach that tries to provide a detailed framework of method variables and integrate robustness into methods throughout development instead of to assessing it at the end. Consequently, it simplifies method development by focusing on statistical theories and experimental strategies for risk evaluation, variable screening, and adjustment 28,29 . .…”

Section: Introductionmentioning

confidence: 99%

Response surface experimental design for simultaneous chromatographic determination of two antiviral agents “Favipiravir and Remdesivir” in pharmaceuticals and spiked plasma samples

Abdel Hakiem,

Boushra,

Noureldeen

et al. 2024

Journal of Chemometrics

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The antiviral agents, Favipiravir (FAV) and Remdesivir (REM), were introduced in the last few years alone or as combination regimen for successful management of the rapidly spreading CORONA virus pandemic. A newly developed rapid and sensitive high performance liquid chromatographic method (HPLC) has been developed for the simultaneous determination of their mixture. Firstly, one factor at a time optimization (OFAT) has been applied. Afterwards, quality by design approach (QbD) has been utilized using Box Behnken experimental design (BBD) for the development of an experimental design of four independent and nine dependent variables for much better refining of the optimized parameters. The established model has given an optimum resolution at; acetonitrile percentage of 52.66, mobile phase of pH 2.91, percentage of triethylamine of 0.15 and 1.30 mL/min flow rate. The proposed method has been validated according to the USP 31 NF 26 guidelines. Good linearity ranges have been obtained from 5.00 up to 50.00 μg/mL for FAV and from 2.00 up to 60.00 μg/mL for FAV and REM, respectively. Excellent relative standard deviation values (not more than 1.40) were obtained upon investigation of accuracy, precision and robustness. The developed method has succeeded in analysis of investigated drugs in their pharmaceutical formulations and spiked plasma samples with good recoveries of 99.00 and up to 106.00%. The proposed method is considered eligible for the quality control laboratories as well as in‐vivo determinations of both analytes.

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Section: Introductionmentioning

confidence: 99%

Response surface experimental design for simultaneous chromatographic determination of two antiviral agents “Favipiravir and Remdesivir” in pharmaceuticals and spiked plasma samples

Abdel Hakiem,

Boushra,

Noureldeen

et al. 2024

Journal of Chemometrics

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“…At least 20 β-blockers are now commonly used, e.g., metoprolol, atenolol, propranolol, alprenolol, carvedilol, etc. [ 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. On the other hand, long-term treatment with β-blockers might induce depression and consequently the risk of suicide [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…In most cases, separation has been performed on alkyl stationary phases, such as C18 and C8. The cyano stationary phases and unmodified silica-based columns have also been used [ 4 , 5 , 6 , 9 , 11 , 12 ], as well as monolithic ones [ 7 ]. Mobile phases applied to β-blocker analysis usually consist of different combinations of acetonitrile or methanol with buffers.…”

Section: Introductionmentioning

confidence: 99%

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Beta-Blocker Separation on Phosphodiester Stationary Phases—The Application of Intelligent Peak Deconvolution Analysis

et al. 2023

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Beta-blockers are a class of medications predominantly used to manage abnormal heart rhythms. They are also widely used to treat high blood pressure. From the liquid chromatography separation point of view, beta-blockers are interesting molecules due to their hydrophobic–hydrophilic properties. Thus, the study aimed to investigate the beta-blocker separation selectivity on four phosphodiester stationary phases in reversed-phase liquid chromatography (RP LC) and hydrophilic interactions liquid chromatography (HILIC). On tested stationary phases, beta-blockers provide retention in both chromatographic systems, RP LC and HILIC. Additionally, it was found that cation-exchange mechanisms have a significant contribution to retention. Separations were enhanced by applying ChromSword software for gradient optimization and Intelligent Peak Deconvolution Analysis to separate unseparated peaks digitally.

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Chemometrics of anisotropic lipophilicity of anticancer androstane derivatives determined by reversed-phase ultra high performance liquid chromatography with polar aprotic and protic modifiers

Kоvаčеvić

Banjac²,

Anojčić³

et al. 2022

Journal of Chromatography A

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In depth investigation of the retention behavior of structurally related β-blockers on RP-HPLC column: Quality by design and quantitative structure-property relationship complementary approaches for optimization and validation

Cited by 7 publications

References 39 publications

Response surface experimental design for simultaneous chromatographic determination of two antiviral agents “Favipiravir and Remdesivir” in pharmaceuticals and spiked plasma samples

Response surface experimental design for simultaneous chromatographic determination of two antiviral agents “Favipiravir and Remdesivir” in pharmaceuticals and spiked plasma samples

Beta-Blocker Separation on Phosphodiester Stationary Phases—The Application of Intelligent Peak Deconvolution Analysis

Chemometrics of anisotropic lipophilicity of anticancer androstane derivatives determined by reversed-phase ultra high performance liquid chromatography with polar aprotic and protic modifiers

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