2022
DOI: 10.1038/s41467-022-35367-2
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In-depth mapping of protein localizations in whole tissue by micro-scaffold assisted spatial proteomics (MASP)

Abstract: Accurate, in-depth mapping of proteins on whole-tissue levels provides comprehensive insights into the spatially-organized regulatory processes/networks in tissues, but is challenging. Here we describe a micro-scaffold assisted spatial proteomics (MASP) strategy, based on spatially-resolved micro-compartmentalization of tissue using a 3D-printed micro-scaffold, capable of mapping thousands of proteins across a whole-tissue slice with excellent quantitative accuracy/precision. The pipeline includes robust tissu… Show more

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Cited by 17 publications
(12 citation statements)
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“…Distribution of the proteins and their corresponding abundance on mouse brain was reported by other laboratories. Although the excision area in these studies are diverse (our study reached the minimized size of 0.05 mm 2 in mouse brain), and the spatial views to some brain proteins are different, all the studies attain a consistent conclusion that these proteins related to brain functions present a characteristic of spatial distribution, implying the protein abundance closely associated with the specific zones of brain. , From our database, 20 mouse brain proteins were selected, and the spatial map of the individual proteins were construction as indicated in Figure S7. In short, the integrated analysis of protein abundance clustering, regional abundance correlation, and functional enrichment in the anatomic zone enables the construction of a preliminary proteomics atlas in the mouse brain.…”
Section: Resultsmentioning
confidence: 87%
See 1 more Smart Citation
“…Distribution of the proteins and their corresponding abundance on mouse brain was reported by other laboratories. Although the excision area in these studies are diverse (our study reached the minimized size of 0.05 mm 2 in mouse brain), and the spatial views to some brain proteins are different, all the studies attain a consistent conclusion that these proteins related to brain functions present a characteristic of spatial distribution, implying the protein abundance closely associated with the specific zones of brain. , From our database, 20 mouse brain proteins were selected, and the spatial map of the individual proteins were construction as indicated in Figure S7. In short, the integrated analysis of protein abundance clustering, regional abundance correlation, and functional enrichment in the anatomic zone enables the construction of a preliminary proteomics atlas in the mouse brain.…”
Section: Resultsmentioning
confidence: 87%
“…Although the excision area in these studies are diverse (our study reached the minimized size of 0.05 mm 2 in mouse brain), and the spatial views to some brain proteins are different, all the studies attain a consistent conclusion that these proteins related to brain functions present a characteristic of spatial distribution, implying the protein abundance closely associated with the specific zones of brain. 32,33 From our database, 20 mouse brain proteins were selected, and the spatial map of the individual proteins were construction as indicated in Figure S7.…”
Section: Assessment Of He Staining Interferences In Proteinmentioning
confidence: 99%
“…Spatially resolved proteomics based on various microdissection techniques has made tremendous progress in profiling of thousands of proteins while preserving spatial information [7][8][9][10] . Guided by hematoxylin-eosin (H&E)-or immunohistochemical (IHC)-stained images, laser microdissection (LMD)-based spatial proteomics has been successfully demonstrated in providing unprecedented insights into the heterogeneous tissue context of lethal disease tissue samples with cell-type resolution.…”
Section: Mainmentioning
confidence: 99%
“…Additionally, improvements in multiplexing protein/peptide labeling and fast liquid chromatography (LC) separations further improve the throughput, allowing for large-scale proteomic analysis within reasonable instrument time 25, 26, 27 . Boosted by these advances, bottom-up proteomics has been applied to profile protein abundance on tissue sections with deep proteome coverages and high spatial resolution 28, 29, 30, 31, 32, 33 . In most of spatial proteomics workflows, sample isolation was performed by laser microdissection (LMD or LCM).…”
Section: Introductionmentioning
confidence: 99%