In depth understanding the molecular response to the enhanced secretion of fatty acids in S accharomyces cerevisiae due to one-step gene deletion of acyl-CoA synthetases

Fang, Fang; Dai, Bingye; Zhao, Guang; Zhao, Hua; Sun, Chao; Liu, Hui

doi:10.1016/j.procbio.2016.05.017

Cited by 6 publications

(3 citation statements)

References 51 publications

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“…Several oxidoreductases were also variably up- or down-regulated in samples including afr (N007_RS24445), cbaA (N007_RS24560), sll1783 (N007_RS25800), SH2032 (N007_RS25355), Mflv _1521 (N007_RS38830), and gno (N007_RS31205) genes (Figure B). A similar regulation of these oxidoreductases has been observed as a stress response in Saccharomyces cerevisiae, Mycobacterium tuberculosis, Ruminococcus gauvreauii, and Veronica Guariglia-Oropeza strains. − …”

Section: Resultssupporting

confidence: 65%

Transcriptomic Analysis of the Molecular Mechanisms Underlying the Antibacterial Activity of IONPs@pDA-Nisin Composites toward Alicyclobacillus acidoterrestris

Song

Niu

et al. 2019

ACS Appl. Mater. Interfaces

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A simple and no-drug resistance antibacterial method was developed by the synthesis of heat-stable and pH-tolerant nisin-loaded iron oxide nanoparticles polydopamine (IONPs@pDA) composites. The composites had a crystal structure and diameters of 25 ± 3 nm, with a saturation magnetization (M s) of 43.7995 emu g–1. Nisin was successfully conjugated onto the IONPs@pDA nanoparticles, as evinced by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analyses. The novel synthesized material showed good performance in reducing Alicyclobacillus acidoterrestris, a common food spoilage bacterium that represents a significant problem for the food industry. Treatment of A. acidoterrestris cells with composites resulted in membrane damage, as observed by live/dead staining and scanning electron microscopy and transmission electron microscopy analyses. Further, the composites exhibited highly efficient antibacterial activity against cells in only 5 min. Transcriptomic sequencing of culture RNA pools after exposure to composites resulted in a total of 334 differentially expressed genes that were primarily associated with transcriptional regulation, energy metabolism, membrane transporters, membrane and cell wall syntheses, and cell motility. Thus, these results suggested that changes in transcriptional regulation caused by aggregated composites on target cells led to major changes in homeostasis that manifested by decreased energy metabolism, pore formation in the membrane, and repressed cell wall synthesis. Concomitantly, cell motility and sporulation activities were both repressed, and finally, intracellular substances flowed out of leaky cells. The proposed biocontrol method represents a novel means to control microorganisms without inducing drug resistance. Further, these results provide novel insights into the molecular mechanisms underlying the antibacterial activity of composites against microorganisms.

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Section: Resultssupporting

confidence: 65%

Transcriptomic Analysis of the Molecular Mechanisms Underlying the Antibacterial Activity of IONPs@pDA-Nisin Composites toward Alicyclobacillus acidoterrestris

Song

Niu

et al. 2019

ACS Appl. Mater. Interfaces

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“…Some proteins involved in the export of fatty acids in S. cerevisiae have been described. An omics analysis of fatty acid secreting mutants of S. cerevisiae identified potential fatty acid export protein Mrp8, though no experimental results are available for this transporter (Fang et al, 2016). An experimentally proven fatty acid export protein is Tpo1, from the MFS superfamily, that was initially identified as a polyamine transporter involved in the resistance of yeast toward spermidine (Albertsen et al, 2003; Tomitori et al, 1999).…”

Section: Fatty Acid Transport In Yeastmentioning

confidence: 99%

Microbial fatty acid transport proteins and their biotechnological potential

López

Bogaert

2021

Biotech & Bioengineering

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Fatty acid metabolism has been widely studied in various organisms. However, fatty acid transport has received less attention, even though it plays vital physiological roles, such as export of toxic free fatty acids or uptake of exogenous fatty acids.Hence, there are important knowledge gaps in how fatty acids cross biological membranes, and many mechanisms and proteins involved in these processes still need to be determined. The lack of information is more predominant in microorganisms, even though the identification of fatty acids transporters in these cells could lead to establishing new drug targets or improvements in microbial cell factories. This review provides a thorough analysis of the current information on fatty

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“…This pathway is not tightly regulated by nitrogen concentration [114]. Together with glycolysis, it can contribute significantly to the energy supply of resting cells [115]. At the stationary phase, a higher expression of cytochrome P450 monoxygenases [104] and lipid remobilization [40] are also expected.…”

Section: Discussionmentioning

confidence: 99%

Combinatorial Engineering of Yarrowia lipolytica as a Promising Cell Biorefinery Platform for the de novo Production of Multi-Purpose Long Chain Dicarboxylic Acids

2017

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This proof-of-concept study establishes Yarrowia lipolytica (Y. lipolytica) as a whole cell factory for the de novo production of long chain dicarboxylic acid (LCDCA-16 and 18) using glycerol as the sole source of carbon. Modification of the fatty acid metabolism pathway enabled creating a pool of fatty acids in a β-oxidation deficient strain. We then selectively upregulated the native fatty acid ω-oxidation pathway for the enhanced terminal oxidation of the endogenous fatty acid precursors. Nitrogen-limiting conditions and leucine supplementation were employed to induce fatty acid biosynthesis in an engineered Leu -modified strain. Our genetic engineering strategy allowed a minimum production of 330 mg/L LCDCAs in shake flask. Scale up to a 1-L bioreactor increased the titer to 3.49 g/L. Our engineered yeast also produced citric acid as a major by-product at a titer of 39.2 g/L. These results provide basis for developing Y. lipolytica as a safe biorefinery platform for the sustainable production of high-value LCDCAs from non-oily feedstock.

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In depth understanding the molecular response to the enhanced secretion of fatty acids in S accharomyces cerevisiae due to one-step gene deletion of acyl-CoA synthetases

Cited by 6 publications

References 51 publications

Transcriptomic Analysis of the Molecular Mechanisms Underlying the Antibacterial Activity of IONPs@pDA-Nisin Composites toward Alicyclobacillus acidoterrestris

Transcriptomic Analysis of the Molecular Mechanisms Underlying the Antibacterial Activity of IONPs@pDA-Nisin Composites toward Alicyclobacillus acidoterrestris

Microbial fatty acid transport proteins and their biotechnological potential

Combinatorial Engineering of Yarrowia lipolytica as a Promising Cell Biorefinery Platform for the de novo Production of Multi-Purpose Long Chain Dicarboxylic Acids

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