In-fiber optofluidic online SERS detection of trace uremia toxin

Abstract

In this Letter, we propose a microstructured in-fiber optofluidic surface-enhanced Raman spectroscopy (SERS) sensor for the initial inspection of uremia through the detection of unlabeled urea and creatinine. As a natural microfluidic device, microstructured hollow fiber has a special structure inside. Through chemical bonds, the SERS substrate can be modified and grown on the surface of the suspended core. Here, the silver nanoparticles (Ag NPs) are embedded on the poly diallyl dimethyl ammonium chloride–modified graphene oxide sheet to achieve the self-assembled SERS substrate. The reduced distance between Ag NPs can increase the strong hot spots that generate enhanced Raman signals. Therefore, it can effectively detect the Raman signal of unlabeled trace uremic toxin analytes (urea, creatinine) inside the optical fiber. The results show that under simulated biophysical conditions, the limit detection (LOD) for urea is 10 − 4 M and the linearity is good, especially at the clinical conventional concentration range ( 2.5 − 6.5 × 10 − 3 M ). In addition, the online Raman detection of creatinine aqueous solution LOD is 10 − 6 M , which also has good linearity. Significantly, this Letter provides a microstructured optofluidic in-fiber Raman sensor for the preliminary detection of uremia, which will have good development prospects in the field of clinical biomedicine.

Study on Nursing Effect of Psychological Intervention on Uremic Hemodialysis Patients

“…Uremia is a series of poisoning symptoms when acute or chronic renal insufficiency develops to a serious stage, often accompanied by water, electrolyte disorder, endocrine dysfunction, and other symptoms, which poses a serious threat to the life safety of patients [ 14 – 16 ]. Hemodialysis is one of the important treatment methods for patients with uremia, which can effectively improve the survival rate of patients.…”

A Wearable Respiration Sensor for Real-Time Monitoring of Chronic Kidney Disease

“…1# and 2# are uremia patients free from hemodialysis, and 3# and 4# are uremia patients who have undergone multiple hemodialysis, and their body temperature is within the normal range of human body temperature to exclude the influence of temperature on the sensor . Previously studies have shown that the respiratory ammonia and blood urea levels of patients with CKD can be effectively reduced after hemodialysis. − As shown in Figure a and Video S1, the clinical tests were performed with the as-prepared sensor device (Figure b,c). With the extension of breathing time, the current signals increase first, which is caused by the gradual increase of humidity.…”

Optical Biomedical Diagnostics Using Lab-on-Fiber Technology: A Review

“…In the study, target urea sample interacts with the urease enzyme producing ammonia which diffuses through the membrane into a solution of the fluorescent pH indicator resulting in the change in the fluorescence signal. An in-fiber opto-fluidic SERS based sensor for uremia (urea and creatinine) detection was proposed by Gao et al [144]. The sensor was designed by incorporating a thin microfluidic channel inside microstructured hollow-core fiber, while GO/Ag nanoparticles were immobilized inside the hollow core to achieve plasmonic SERS enhancement.…”