In-Field and Early Detection of Xylella fastidiosa Infections in Olive Using a Portable Instrument

Martinelli, Federico; Marchese, Annalisa; Giovino, Antonio; Marra, Francesco Paolo; Noce, Isabella Della; Caruso, Tiziano; Dandekar, Abhaya M.

doi:10.3389/fpls.2018.02007

Cited by 9 publications

(11 citation statements)

References 22 publications

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“…In addition, in situ presymptomatic detection of the bacterium could potentially be performed by a portable instrument that analyzes mRNA of plant genes linked to OQDS (ie, biomarkers) using an in-field qRT-PCR assay. 11 In this case, the detection tool is strictly related to host response at the transcript level upon infection. If the field reliability of such techniques is confirmed by parallel analyses performed using real-time PCR, it would be possible to screen a larger number of trees per plot, thus obtaining more robust results.…”

Section: Discussionmentioning

confidence: 99%

An Evaluation of Monitoring Surveys of the Quarantine Bacterium Xylella Fastidiosa Performed in Containment and Buffer Areas of Apulia, Southern Italy

Scortichini¹,

Cesari

2019

Appl Biosaf.

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Introduction: Xylella fastidiosa is a quarantine phytopathogen for the European Plant Protection Organization and currently infects olive trees in the Apulia region (southern Italy). Upon the Implementing Decision of the European Union 2016/764 of May 12, 2016, extensive monitoring surveys were performed on approximately 190 000 ha to ascertain the possible occurrence of X. fastidiosa. Objectives: The primary objectives of the analysis were to start to collect epidemiological data on X. fastidiosa occurrence in areas far from the initial outbreaks and discuss the results of the pathogen detection. Methods: A total of 220 279 olive trees were inspected. Basic information on farm and trees management was obtained. A total of 13 706 olive trees were analyzed through serological and molecular techniques to verify the possible occurrence of the bacterium. Results: The cultivars “Nociara,” “Cima di Melfi,” and “Cellina di Nardò” showed the highest occurrence of decline symptoms. Tree age appears to be related to the incidence of decline symptoms. Olive trees growing in well-managed soils showed fewer symptoms than trees cultivated in farms where such agronomic techniques are not regularly performed. X. fastidiosa was detected in 2078 samples taken from symptomatic trees and 1653 samples obtained from asymptomatic trees. In 3300 samples taken from symptomatic trees, the bacterium was not detected. Conclusions: Implementation and utilization of reliable in situ detection techniques could increase the number of sampled trees in each plot, thus allowing a more extensive and robust assessment of X. fastidiosa–infected plants in areas where the pathogen inoculums are still low.

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Section: Discussionmentioning

confidence: 99%

An Evaluation of Monitoring Surveys of the Quarantine Bacterium Xylella Fastidiosa Performed in Containment and Buffer Areas of Apulia, Southern Italy

Scortichini¹,

Cesari

2019

Appl Biosaf.

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“…Here, we propose the employment of an instrument that is already widely used for plant pathogens 6,7 and could be easily adapted also for the detection of SARS‐CoV‐2. The idea is to use a portable DNA‐based detection devices not only targeting viral RNA but also analyze a pool of early responsive host transcripts.…”

Section: Biomarker Identificationmentioning

confidence: 99%

“…This miniaturized instrument has been recently proposed for highly threatening pathogen outbreak in agriculture such as Xylella fastidiosa in olive 6 . The instrument has typically been used for the detection of plant viral infections directly in field.…”

Section: Portable Instrument For Coronavirus Infield Detectionmentioning

confidence: 99%

Application of a portable instrument for rapid and reliable detection of SARS‐CoV‐2 infection in any environment

Martinelli

Perrone

Noce³

et al. 2020

Immunological Reviews

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The ongoing outbreak of the novel coronavirus (SARS‐CoV‐2) infection is creating serious challenges for health laboratories that seek to identify viral infections as early as possible, optimally at the earliest appearance of symptom. Indeed, there is urgent need to develop and deploy robust diagnostic methodologies not only to use in health laboratory environments but also directly in places where humans circulate and spread the virus such as airports, trains, boats, and any public aggregation places. The success of a reliable and sensitive asymptomatic diagnosis relies on the identification and measurement of informative biomarkers from human host and virus in a rapid, sensitive, and inexpensive manner. The objective of this article is to describe an innovative multidisciplinary approach to develop an efficient, inexpensive, and easy‐to‐use portable instrument (bCUBE® by Hyris Ltd) that can be employed as a surveillance system for the emergency caused by SARS‐CoV‐2. A solution for Coronavirus testing, compliant with CDC guidelines, is scheduled to be released in the next weeks. In addition, we will describe a workflow and path of an integrated multi‐omic approach that will lead to host and pathogen biomarker discovery in order to train the instrument to provide reliable results based on a specific biomarker's fingerprint of SARS‐CoV‐2 infection.

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“…However, a large number of diseases and disorders affect this crop, mostly caused by fungi, such as Arthrinium phaeospermum , Phoma cladoniicola and Ulocladium consortiale , recently discovered as new olive pathogens in Italy [ 10 ], but also by systemic pathogens including bacteria and viruses, which provoke significant yield losses. Indeed, in the last decade, olive production has suffered an enormous decline due to the emergence of biotic agents that have significantly undermined the Mediterranean economy related to olive and the olive oil industry; a dramatic example being the Xylella fastidiosa epidemic in 2013, which decimated olive trees in Apulia [ 11 ] and created huge losses for the local olive economy and oil production outputs, posing critical challenges for its management, as well as dramatic changes in the landscape [ 12 , 13 ]. Furthermore, the vegetative propagation of olive trees using cuttings of semi-wood has contributed over the years to the spread of systemic-pathogens, particularly viruses [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a Real-Time Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Olea Europaea Geminivirus

Bertacca

Caruso

Trippa

et al. 2022

Plants

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A real-time loop-mediated isothermal amplification (LAMP) assay was developed for simple, rapid and efficient detection of the Olea europaea geminivirus (OEGV), a virus recently reported in different olive cultivation areas worldwide. A preliminary screening by end-point PCR for OEGV detection was conducted to ascertain the presence of OEGV in Sicily. A set of six real-time LAMP primers, targeting a 209-nucleotide sequence elapsing the region encoding the coat protein (AV1) gene of OEGV, was designed for specific OEGV detection. The specificity, sensitivity, and accuracy of the diagnostic assay were determined. The LAMP assay showed no cross-reactivity with other geminiviruses and was allowed to detect OEGV with a 10-fold higher sensitivity than conventional end-point PCR. To enhance the potential of the LAMP assay for field diagnosis, a simplified sample preparation procedure was set up and used to monitor OEGV spread in different olive cultivars in Sicily. As a result of this survey, we observed that 30 out of 70 cultivars analyzed were positive to OEGV, demonstrating a relatively high OEGV incidence. The real-time LAMP assay developed in this study is suitable for phytopathological laboratories with limited facilities and resources, as well as for direct OEGV detection in the field, representing a reliable method for rapid screening of olive plant material.

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In-Field and Early Detection of Xylella fastidiosa Infections in Olive Using a Portable Instrument

Cited by 9 publications

References 22 publications

An Evaluation of Monitoring Surveys of the Quarantine Bacterium Xylella Fastidiosa Performed in Containment and Buffer Areas of Apulia, Southern Italy

An Evaluation of Monitoring Surveys of the Quarantine Bacterium Xylella Fastidiosa Performed in Containment and Buffer Areas of Apulia, Southern Italy

Application of a portable instrument for rapid and reliable detection of SARS‐CoV‐2 infection in any environment

Development of a Real-Time Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Olea Europaea Geminivirus

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