In focus in HCB

Taatjes, Douglas J.; Roth, Jürgen

doi:10.1007/s00418-017-1615-z

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…Nevertheless, some functions are attributed specifically to the ARF6 retrogene. It has been recently demonstrated that it plays an important role in cell-cell interactions (adhesiveness) [65]. Interestingly, a number of studies indicate a strong association of ARF6 protein with human immunodeficiency virus type 1 (HIV-1) [66,67].…”

Section: Known Protein-coding Retrogenesmentioning

confidence: 99%

Complex Analysis of Retroposed Genes’ Contribution to Human Genome, Proteome and Transcriptome

Kubiak

Szcześniak

Makałowska

2020

Genes

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Gene duplication is a major driver of organismal evolution. One of the main mechanisms of gene duplications is retroposition, a process in which mRNA is first transcribed into DNA and then reintegrated into the genome. Most gene retrocopies are depleted of the regulatory regions. Nevertheless, examples of functional retrogenes are rapidly increasing. These functions come from the gain of new spatio-temporal expression patterns, imposed by the content of the genomic sequence surrounding inserted cDNA and/or by selectively advantageous mutations, which may lead to the switch from protein coding to regulatory RNA. As recent studies have shown, these genes may lead to new protein domain formation through fusion with other genes, new regulatory RNAs or other regulatory elements. We utilized existing data from high-throughput technologies to create a complex description of retrogenes functionality. Our analysis led to the identification of human retroposed genes that substantially contributed to transcriptome and proteome. These retrocopies demonstrated the potential to encode proteins or short peptides, act as cis- and trans- Natural Antisense Transcripts (NATs), regulate their progenitors’ expression by competing for the same microRNAs, and provide a sequence to lncRNA and novel exons to existing protein-coding genes. Our study also revealed that retrocopies, similarly to retrotransposons, may act as recombination hot spots. To our best knowledge this is the first complex analysis of these functions of retrocopies.

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Section: Known Protein-coding Retrogenesmentioning

confidence: 99%

Complex Analysis of Retroposed Genes’ Contribution to Human Genome, Proteome and Transcriptome

Kubiak

Szcześniak

Makałowska

2020

Genes

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“…Common technical problems in immunostaining protocols relate to poor signal-tonoise ratios, either due to high background (autofluorescence or unspecific activity in enzyme-based detection systems), or due to aldehyde reactivity of the chemical fixation of the specimens (Rosas-Arellano et al, 2016;Taatjes and Roth, 2017). Accordingly, there has been sustained interest in improving immunolabeling protocols at every step, namely sample fixation, membrane permeabilization, antibody incubation and detection (Bondza-Kibangou et al, 2001;Gosselin et al, 1986;Hagedorn et al, 2006;Huang et al, 1976;Imam et al, 1995;Rosas-Arellano et al, 2018;Schnell et al, 1999;Shi et al, 1991;Waldvogel et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

A Low Cost Antibody Signal Enhancer Improves Immunolabeling in Cell Culture, Primate Brain and Human Cancer Biopsy

et al. 2020

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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In focus in HCB

Cited by 2 publications

References 11 publications

Complex Analysis of Retroposed Genes’ Contribution to Human Genome, Proteome and Transcriptome

Complex Analysis of Retroposed Genes’ Contribution to Human Genome, Proteome and Transcriptome

A Low Cost Antibody Signal Enhancer Improves Immunolabeling in Cell Culture, Primate Brain and Human Cancer Biopsy

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