In honor of Professor Giovanni Fadda: a fighter against tuberculosis

Zanetti, Stefania Anna Lucia; Sechi, Leonardo Antonio; Molicotti, Paola; Delogu, Giovanni; Rubino, Salvatore

doi:10.3855/jidc.3499

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…Of the 8.8 million cases of TB in 2010, 5.7 million were diagnosed and reported to national TB control programmers (NTPs). Among notified cases, there were an estimated 290,000 cases of multidrug-resistant TB (MDR-TB), of which only 53,000 (18%) were reported to have been diagnosed and enrolled in appropriate treatment [3]. Earlier and improved detection of TB and expanded capacity to diagnose cases of MDR-TB are thus global priorities for TB control.…”

Section: Introductionmentioning

confidence: 99%

Cost-effectiveness in the diagnosis of tuberculosis: choices in developing countries

Molicotti

Bua

Zanetti

2014

J Infect Dev Ctries

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Tuberculosis remains one of the major causes of global death from a single infectious agent. This situation is worsened by the HIV/AIDS pandemic because one-third of HIV/AIDS patients are co-infected with Mycobacterium tuberculosis. Failure to control the spread of tuberculosis is largely due to our inability to detect and treat all infectious cases of pulmonary tuberculosis in a timely manner, allowing continued M. tuberculosis transmission within communities. Diagnosis of tuberculosis can be made using indirect and direct methods. The indirect tests, such as interferon-gamma release assays, provide a new diagnostic method for M. tuberculosis infection, but do not discriminate between infection and active disease. The most common direct method for diagnosing TB worldwide is sputum smear microscopy (developed more than 100 years ago), where bacteria are observed in sputum samples examined under a microscope. In countries with more developed laboratory capacities, cases of tuberculosis may also be diagnosed using culture methods (the current gold standard) or, increasingly, using rapid molecular tests. In this review, we discuss the traditional methods for the diagnosis of tuberculosis. We also discuss other inexpensive assays that can be used to detect the presence of M. tuberculosis.

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Section: Introductionmentioning

confidence: 99%

Cost-effectiveness in the diagnosis of tuberculosis: choices in developing countries

Molicotti

Bua

Zanetti

2014

J Infect Dev Ctries

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Comparison of LCD array and IS6110-PCR with conventional techniques for detection of Mycobacterium bovis isolated from Egyptian cattle and Buffaloes

Zahran¹,

Behiry²,

Marzouk³

et al. 2014

International Journal of Mycobacteriology

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Bovine tuberculosis is a chronic bacterial and major infectious disease of cattle and buffaloes caused by Mycobacterium bovis. Rapid diagnosis of bovine tuberculosis is considered one of the cornerstones for worldwide control as it permits early epidemiological and therapeutic interventions. Therefore, this study was designed to evaluate conventional techniques (tuberculin test, Ziehl Neelsen staining and culturing) in comparison with proven molecular laboratory techniques (LCD array and IS6110 PCR) for identification of Bovine tuberculosis. A total of 902 Egyptian animals (480 buffaloes and 422 cattle) were examined by tuberculin test, and the positive reactors were slaughtered. Tissue samples were collected for staining as well as culturing. Moreover, LCD array and PCR using IS6110 on DNA extracted from tissue and culture samples were carried out for molecular identification of M. bovis. According to the results, the tuberculin positive cases for cattle and buffaloes were 2.14% (9 cases) and 5.62% (27 cases), respectively. After post-mortem examination, the prevalence of tuberculin positive cases with visible lesions was 88.9% for cattle and 14.8% for buffaloes. Alternatively, these percentages were 11.1% and 85.2% for cattle and buffalo carcasses with non-visible lesions. The percentage of cattle and buffaloes showing positive culture was 88.9% and 62.9%, respectively. This percentage was 69.5% after staining with Ziehl Neelsen. In contrast, LCD array and IS6110 were 100%, confirming the isolation results. In conclusion, LCD array depending on 16S RNA and DNA hybridization with specific probes for detection of M. bovis are rapid, sensitive and labor-saving when combined with IS6110-PCR.

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In honor of Professor Giovanni Fadda: a fighter against tuberculosis

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Cited by 2 publications

References 9 publications

Cost-effectiveness in the diagnosis of tuberculosis: choices in developing countries

Cost-effectiveness in the diagnosis of tuberculosis: choices in developing countries

Comparison of LCD array and IS6110-PCR with conventional techniques for detection of Mycobacterium bovis isolated from Egyptian cattle and Buffaloes

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