In house synthesized novel distyryl-BODIPY dye and polymer assembly as deep-red emitting probe for protamine detection

A Kumujian-C based highly selective fluorescence turn-on probe enables the detection of sulfite in real samples and living cells

“…The high polarity of the solvent aids this electron delocalization, significantly stabilising the dye, which ultimately favours shifting of its absorption maxima towards longer wavelengths. 16,36,37 The emission spectra of the dye also followed a similar trend (cf. Fig.…”

“…and above all, this can be useful for in vivo sensing. [16][17][18] Thus, a considerable amount of effort is still being given to developing fluorescence-based (on-off, off-on, ratiometric) sensors for the detection of SO 2 and its derivatives. All these sensors detect SO 3 2À /HSO 3 À based on nucleophilic reaction with aldehyde, 19,20 deprotection of levulinate, 10,21 Michael addition reaction, 22,23 coordination to metal ions, etc.…”

“…From this, the LOD for sulfite was calculated to be B0.35 mM, using IUPAC recommended formula 3s/S, where s corresponds to the standard deviation of 10 successive measurements of 15 mM Kum-C in phosphate buffer without any sulfite ions and 'S' is the slope of the linearity plot. 16,17 It is imperative to mention here that although the reaction between the aldehyde and sulfite apparently looks irreversible, the reaction is actually reversible in nature, since the aldehyde compound can easily be recovered from alkaline or acidic medium. 41,42 Thus, in the presence of external stimuli, the adduct can be dissociated to get the initial product unharmed.…”

A simplified and cost-effective detection of cancer bio-markers using BODIPY and surfactant-templated fluorogenic self-assembly

“…Furthermore, we also tried spermine quantification with the BDP-Sty@PSS complex, which was used in our previous study for protamine detection. 31 It is found that although the BDP-Sty@SDS complex appreciably recognizes Sp, the sensitivity of this complex towards Sp is significantly less as compared to BDP-Sty@SDS assembly ( cf. S4, ESI†).…”

“…Previously designed, synthesized, purified and characterized BODIPY distyryl (abbreviated as BDP-Sty) as reported in our earlier work, 31 has been used in the present study. Sodium dodecyl sulfate (SDS), cetyltrimethylammonium bromide (CTAB), inorganic salts such as NaCl, KCl, CaCl 2 , ZnCl 2 , HgCl 2 , CuSO 4 and Al(NO 3 ) 3 along with bio-analytes, viz ., glucose, di-sodium salt of adenosine-triphosphate (ATP), and chloride salts of bio-amines (cadaverine, putrescine, spermidine, spermine) were obtained from Sisco research laboratory (SRL), India and utilized without any further purification.…”