Transvaginal hydrolaparoscopy is performed to investigate tubal pathology in subfertile women. We studied the findings at THL and subsequent pregnancy rates in a retrospective multicenter cohort study. Between 2000 and 2011 1,033 subfertile women participated in our study. Primary outcome measure was intra-uterine pregnancy, either after natural conception or after treatment with intra-uterine insemination or ovulation induction. We calculated cumulative intra-uterine pregnancy rates using Kaplan-Meier analysis and fecundity rate ratios (FRR). THL showed bilateral patent tubes in 83%, one-sided tubal occlusion in 12.4% and bilateral tubal occlusion in 4.6% of women. Cumulative intrauterine pregnancy rates after 36 months were 52% for women with bilateral patent tubes, 44% for one-sided tubal occlusion (FRR 1.04 (95% CI 0.78-1.39) and 7% for bilateral tubal occlusion (FRR 0.13 (95% CI 0.04-0.43). Endometriosis was diagnosed in 6.4%, adhesions in 9.1%, while 3.9% of women had both. Corresponding FRRs were 0.73 (95% CI 0.49-1.09), 0.68 (95% CI 0.46-1.02), and 0.42 (95% CI 0.20-0.84). In conclusion, women with bilateral tubal occlusion or the combination of endometriosis and adhesions found at THL have significantly reduced chances of natural conception.