In ovulo rescue of hybrid embryos in Flame Seedless grapes—Influence of pre-bloom sprays of benzyladenine

Breeding new seedless grape by means of in vitro embryo rescue

“…The effects of genotype on embryo rescue have been reported in earlier studies (Burger and Goussard, 1996;Liu et al, 2003;Bharathy et al, 2005;Tian et al, 2008;Guo et al, 2010). In the present study, we found that embryo formation, embryo germination, and plant development varied greatly among different cross-combinations.…”

“…In previous studies, the embryo rescue technique was used to breed triploid grapes (Yamashita et al, 1998;Bessho et al, 2000;Bharathy et al, 2005;Tian et al, 2008;Sun et al, 2011). In this study, we set four crosses with 2X x 4X and obtained triploid hybrid seedlings from 'Ruby Seedless x Black Olympia', 'DA7 x Jingyou', and 'Big Black x Kyoho'.…”

“…Earlier in 1982, Ramming and Emershad first reported that stenospermic grapes could generate plants via ovule culture. Since then, this embryo rescue technique has been widely applied to embryo germination of seedless grape cultivars in cross-breeding programs (Emershad and Ramming, 1984;Spiegle-Roy et al, 1985;Gray et al, 1990;Ponce et al, 2000;Bharathy et al, 2005;Tian et al, 2008;Tang et al, 2009;Singh et al, 2011). Nowadays, in vitro embryo rescue in grape breeding projects assures breeding efficiency by curtailing 6 to 8 years in the development of seedless grape cultivars (Singh et al, 2011), and the adoption of seeded cultivars as female parents has been replaced by using stenospermic grapes as females directly.…”

“…In addition, the cross between grapes of different ploidy was also an effective way to obtain new seedless grape cultivars by the embryo rescue technique (Yamashita et al, 1998;Bessho et al, 2000;Bharathy et al, 2005;Tian et al, 2008;Sun et al, 2011). It has been proven that it was easier to harvest hybrid progeny when using diploids as female parents (Guo et al, 2010).…”

Immature Embryo Rescue and <i>in Vitro</i> Development Evaluation of Intraspecific Hybrids from Brazilian Seedless Grapevine “Superior × Thompson” Clones

“…Several studies have demonstrated the importance of determining the best period for the isolation of immature embryos from grapevine crosses. In a study by [13], using embryo rescue technique for the "Flame Seedless" vine variety, it was obtained (100%) of embryos, but with a smaller capacity (<20%) of generating seedlings. Reference [14] had obtained 20 seedlings from 44 rescued embryos from the interspecific cross between V. vinifera and V. rotundifolia.…”