In planta Genome Editing in Commercial Wheat Varieties

Liu, Yuelin; Luo, Weifeng; Linghu, Qianyan; Abe, Fumitaka; Hisano, Hiroshi; Sato, Kazuhiro; Kamiya, Yoko; Kawaura, Kanako; Onishi, Kazumitsu; Endo, Masaki; Toki, Seiichi; Hamada, Hideaki; Nagira, Yozo; Taoka, Naoaki; Imai, Ryozo

doi:10.3389/fpls.2021.648841

Cited by 36 publications

(23 citation statements)

References 19 publications

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“…1d). Collectively, these results demonstrated that the iPB-RNP method is capable of being deployed for in planta genome editing with comparable efficiency to the iPB-DNA method 9 .…”

Section: Mainmentioning

confidence: 78%

In planta genome editing with CRISPR/Cas9 ribonucleoproteins

Kumagai

Hamada

et al. 2021

Preprint

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In planta genome editing represents an attractive approach to engineering crops/varieties that are recalcitrant to culture-based transformation methods. Here, we report the direct delivery of CRISPR/Cas9 ribonucleoproteins into the shoot apical meristem using in planta particle bombardment and introduction of a semidwarf1 (sd1)-orthologous mutation into wheat. The triple knockout tasd1 mutant of an elite wheat variety reduced culm length by 10% without a reduction in yield.

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“…1d). Collectively, these results demonstrated that the iPB-RNP method is capable of being deployed for in planta genome editing with comparable efficiency to the iPB-DNA method 9 .…”

Section: Mainmentioning

confidence: 78%

In planta genome editing with CRISPR/Cas9 ribonucleoproteins

Kumagai

Hamada

et al. 2021

Preprint

Self Cite

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“…Generally, winter cultivars are more difficult to transform as they require vernalisation and the transformation rates are lower compared to spring varieties and, as part of the study, we optimised the transformation procedure for them (see Materials and Methods). Since there are only few published examples of genome editing in winter wheat [ 26 , 27 ], our work will be of interest to researchers working on winter wheat transformation and genome editing.…”

Section: Discussionmentioning

confidence: 99%

Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties

Hahn

Loures

Sparks

et al. 2021

Plants

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CRISPR/Cas technology has recently become the molecular tool of choice for gene function studies in plants as well as crop improvement. Wheat is a globally important staple crop with a well annotated genome and there is plenty of scope for improving its agriculturally important traits using genome editing technologies, such as CRISPR/Cas. As part of this study we targeted three different genes in hexaploid wheat Triticum aestivum: TaBAK1-2 in the spring cultivar Cadenza as well as Ta-eIF4E and Ta-eIF(iso)4E in winter cultivars Cezanne, Goncourt and Prevert. Primary transgenic lines carrying CRISPR/Cas-induced indels were successfully generated for all targeted genes. While BAK1 is an important regulator of plant immunity and development, Ta-eIF4E and Ta-eIF(iso)4E act as susceptibility (S) factors required for plant viruses from the Potyviridae family to complete their life cycle. We anticipate the resultant homozygous tabak1-2 mutant lines will facilitate studies on the involvement of BAK1 in immune responses in wheat, while ta-eif4e and ta-eif(iso)4e mutant lines have the potential to become a source of resistance to wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV), both of which are important pathogens of wheat. As winter wheat varieties are generally less amenable to genetic transformation, the successful experimental methodology for transformation and genome editing in winter wheat presented in this study will be of interest to the research community working with this crop.

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“…To generate the ta-eif4e and ta-eif(iso)4e lines, we needed to optimise the transformation procedure for the three winter wheat cultivars Cezanne, Goncourt and Prevert (see Materials and Methods). Since there are only few published examples of genome editing in winter wheat (Li et al, 2020;Liu et al, 2021), our work will be of interest to researchers working on winter wheat transformation and genome editing.…”

Section: Discussionmentioning

confidence: 99%

Efficient CRISPR/Cas-mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties

Hahn

Loures

Sparks

et al. 2021

Preprint

View full text Add to dashboard Cite

The CRISPR/Cas technology has recently become a molecular tool of choice for gene function studies in plants as well as crop improvement. Wheat is a globally important staple crop with a well annotated genome and there is plenty of scope for improving its agriculturally important traits using genome editing technologies, such as CRISPR/Cas. As part of this study we targeted three different genes in hexaploid wheat Triticum aestivum: TaBAK1-2 in the spring cultivar Cadenza as well as Ta-eIF4E and Ta-eIF(iso)4E in winter cultivars Cezanne, Goncourt and Prevert. The primary transgenic lines carrying CRISPR/Cas-induced indels were successfully generated for all targeted genes. As winter wheat varieties are generally less amenable to genetic transformation, the successful experimental methodology for transformation and genome editing in winter wheat presented in this study will be of interest to the research community working with this crop.

show abstract

In planta Genome Editing in Commercial Wheat Varieties

Cited by 36 publications

References 19 publications

In planta genome editing with CRISPR/Cas9 ribonucleoproteins

In planta genome editing with CRISPR/Cas9 ribonucleoproteins

Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties

Efficient CRISPR/Cas-mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties

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