In planta haploid induction by <i>kokopelli</i> mutants

Jacquier, Nathanaël; Calhau, Andrea R M; Fierlej, Yannick; Martinant, Jean‐Pierre; Rogowsky, Peter; Gilles, Laurine; Widiez, Thomas

doi:10.1093/plphys/kiad328

Cited by 7 publications

(3 citation statements)

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“…Altogether, these results suggest a possible involvement of Zm00001d026242 in fertilization and haploid induction. Recently, Jacquier et al. (2023) validated the role of Atkpl (KOKOPELLI, AT5G63720) mutant for triggering in planta maternal haploid induction.…”

Section: Discussionmentioning

confidence: 92%

Genetic basis of maize maternal haploid induction beyond MATRILINEAL and ZmDMP

Trentin,

Krause,

Zunjare

et al. 2023

Front. Plant Sci.

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In maize, doubled haploid (DH) lines are created in vivo through crosses with maternal haploid inducers. Their induction ability, usually expressed as haploid induction rate (HIR), is known to be under polygenic control. Although two major genes (MTL and ZmDMP) affecting this trait were recently described, many others remain unknown. To identify them, we designed and performed a SNP based (~9007) genome-wide association study using a large and diverse panel of 159 maternal haploid inducers. Our analyses identified a major gene near MTL, which is present in all inducers and necessary to disrupt haploid induction. We also found a significant quantitative trait loci (QTL) on chromosome 10 using a case-control mapping approach, in which 793 noninducers were used as controls. This QTL harbors a kokopelli ortholog, whose role in maternal haploid induction was recently described in Arabidopsis. QTL with smaller effects were identified on six of the ten maize chromosomes, confirming the polygenic nature of this trait. These QTL could be incorporated into inducer breeding programs through marker-assisted selection approaches. Further improving HIR is important to reduce the cost of DH line production.

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Section: Discussionmentioning

confidence: 92%

Genetic basis of maize maternal haploid induction beyond MATRILINEAL and ZmDMP

Trentin,

Krause,

Zunjare

et al. 2023

Front. Plant Sci.

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“…Thus, other genes must be responsible for increasing haploid induction ability in our DHIs. Those could be the more recently identified HIR genes (Li et al, 2021;Jiang et al, 2022;Jacquier et al, 2023). However, Trentin et al (2023) and others have shown that there is additional genetic variation available for HIRs, in addition to the four The distribution of parental genome contribution to doubled haploid inducers (DHIs) derived from the 23 biparental crosses.…”

Section: Discussionmentioning

confidence: 99%

“…Additional genome regions and underlying candidate genes have been identified as putatively controlling HIRs in a genome-wide association study (e.g., Trentin et al, 2023). A novel gene in the Arabidopsis model plant, AtKPl (KOKOPELLI), which is only expressed in male gametophytes, has been discovered to play a role in single fertilization events to trigger maternal haploid induction (Jacquier et al, 2023). Although the biological mechanism of haploid induction in maize remains ambiguous, effective methods for accumulating favorable alleles of other genes affecting HIRs in fixed mtl genetic backgrounds are required for further genetic improvement of inducers and biological studies of haploid induction.…”

Section: Introductionmentioning

confidence: 99%

Development of doubled haploid inducer lines facilitates selection of superior haploid inducers in maize

Chen,

Lübberstedt,

Frei

2024

Front. Plant Sci.

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Haploid inducers are key components of doubled haploid (DH) technology in maize. Robust agronomic performance and better haploid induction ability of inducers are persistently sought through genetic improvement. We herein developed C1-I inducers enabling large-scale in vivo haploid induction of inducers and discovered superior inducers from the DH progenies. The haploid induction rate (HIR) of C1-I inducers ranged between 5.8% and 12.0%. Overall, the success rate of DH production was 13% on average across the 23 different inducer crosses. The anthesis–silking interval and days to flowering of inducer F1s are significantly correlated with the success rate of DH production (r = −0.48 and 0.47, respectively). Transgressive segregants in DH inducers (DHIs) were found for the traits (days to flowering, HIR, plant height, and total primary branch length). Moreover, the best HIR in DHIs exceeded 23%. Parental genome contributions to DHI progenies ranged between 0.40 and 0.55, respectively, in 25 and 75 percentage quantiles, and the mean and median were 0.48. The allele frequency of the four traits from inducer parents to DHI progenies did not correspond with the phenotypic difference between superior and inferior individuals in the DH populations by genome-wide Fst analysis. This study demonstrated that the recombinant DHIs can be accessed on a large scale and used as materials to facilitate the genetic improvement of maternal haploid inducers by in vivo DH technology.

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