In planta histochemical staining of fungal alkaline phosphatase activity for analysis of efficient arbuscular mycorrhizal infections

Tisserant, Benoît; Gianinazzi‐Pearson, Vivienne; Gianinazzi, Silvio; Gollotte, Armelle

doi:10.1016/s0953-7562(09)80248-7

Cited by 218 publications

(147 citation statements)

References 16 publications

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“…However, only 2.4% to 3.4% of ERM structures show ACP activity in all treatments. As ALP activity can serve as a marker for AMF metabolic activity [38], the present results may indicate that AMF can maintain some metabolic activities under Cr(VI) contaminations. Besides, when the hyphae was treated with 0, 0.05, 0.1, 0.2, 1.0 mmol L −1 Cr(VI) for 96 h, the pH in the hyphal compartment increased to 6.5, 6.4, 6.2, 6.5, 6.1 respectively without significant differences between each other.…”

Section: Mycorrhizal Development and Hyphal Phosphatase Activitymentioning

confidence: 62%

Chromium immobilization by extra- and intraradical fungal structures of arbuscular mycorrhizal symbioses

Zhang

Sun

et al. 2016

Journal of Hazardous Materials

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Section: Mycorrhizal Development and Hyphal Phosphatase Activitymentioning

confidence: 62%

Chromium immobilization by extra- and intraradical fungal structures of arbuscular mycorrhizal symbioses

Zhang

Sun

et al. 2016

Journal of Hazardous Materials

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“…Indeed, AMF structures appear to grow most rapidly, and transfer C and P most actively, when they are located near a growing root tip (Buwalda et al 1982;Walker and Smith 1984). As the AMF structures age and become more distant from the root tip, activities decline (Tisserant et al 1993;Tisserant et al 1996). Thus, some fraction of intraradical AMF structures may not be actively contributing to plant P uptake, especially where older roots are present (Allen 2001).…”

Section: What Influences Prlc?mentioning

confidence: 99%

The extent of mycorrhizal colonization of roots and its influence on plant growth and phosphorus content

2013

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Aims The most common metric of arbuscular mycorrhizal fungal (AMF) abundance is percent root length colonized (PRLC) by mycorrhizal structures. Frequently, plants with greater PRLC are assumed to receive more nutrients (such as phosphorus, P) from their mycorrhizal symbionts, leading to greater plant growth. Nevertheless, the functional significance of this metric remains controversial. In this review, I discuss whether manipulations of PRLC generally led to changes in plant biomass and P content, and whether AMF taxa and plant functional groups influence these relationships. Methods I conducted a meta-analysis of laboratoryand field-based trials in which mycorrhizal colonization was directly altered compared to unmanipulated controls. For each trial, I calculated (1) the difference in PRLC (ΔPRLC) between the treatments, and (2) the response ratio of plant biomass. In a subset of these studies, the response ratio of P content of host plants could also be calculated. ResultsThe response ratio of plant biomass and P content rose significantly and exponentially as ΔPRLC increased. Nevertheless, ΔPRLC explained only a fraction of the variation in response ratios in each case. Moreover, AMF taxa varied in their effects on biomass per unit ΔPRLC. In addition, plant functional groups differed in effects on plant P content per unit ΔPRLC, with C4 grasses responding most strongly. Conclusions It appears that as the extent to which plant roots are colonized by AMF increases, plant growth and P content often increase, although substantial variability exists among trials. As others have found, a likely mechanism for this relationship is increased transfer of P (and perhaps other nutrients) through the more-prevalent mycorrhizal structures.

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“…This technique decreased the loss of alkaline phosphatase activity that can occur after the extraction of extra-radical mycelium by aqueous membrane filtration (C. L. Boddington, unpublished). The roots and extra-radical mycelium were stained for alkaline phosphatase by using the technique of Tisserant et al (1993), counterstained overnight at room temperature in 0.1 % acid fuchsin in lacto-glycerol and then destained overnight in 50 % glycerol. The root pieces, stained for alkaline phosphatase, were sampled randomly and 10 cmi1 cm lengths were mounted in 50 % glycerol on a microscope slide.…”

Section: Assessment Of Total Colonized Root Length Root Length Contamentioning

confidence: 99%

Evidence that differences in phosphate metabolism in mycorrhizas formed by species of Glomus and Gigaspora might be related to their life‐cycle strategies

Boddington

Dodd

1999

New Phytologist

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A glasshouse experiment was done to assess the development and phosphate metabolism of mycorrhizas formed by species of arbuscular mycorrhizal fungi (AMF) from two different genera, Gigaspora and Glomus on Desmodium ovalifolium plants at three concentrations of a phosphate source. The addition of phosphate (0-100 mg P kg −" ) had no effect on the alkaline phosphatase activity, stained histochemically, in the intra-radical mycelium of Gigaspora rosea (BEG111), but decreased that of Glomus manihotis (BEG112) over a 10-wk period. The alkaline phosphatase activity of the extra-radical mycelium was unaffected by increasing phosphate addition (0-100 mg P kg −" ) in both species of AMF over a 10-wk period. The extra-radical mycelium of Gi. rosea (BEG111) accumulated polyphosphate, determined by staining with 4h,6-diamidino-2-phenylindole, whereas polyphosphate was not detected in the extra-radical mycelium of G. manihotis (BEG112). This work indicates differences in the mechanisms of phosphate metabolism in the mycelium of AMF from different genera on a tropical host. This might be determined by the life-cycle strategies of these fungi, in particular the formation of auxiliary cells in Gigaspora. The possibility of a negative-feedback mechanism between alkaline phosphatase and polyphosphate in the extra-radical mycelium of Gi. rosea (BEG111) and the role of polyphosphate in the symbiosis are discussed.

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In planta histochemical staining of fungal alkaline phosphatase activity for analysis of efficient arbuscular mycorrhizal infections

Cited by 218 publications

References 16 publications

Chromium immobilization by extra- and intraradical fungal structures of arbuscular mycorrhizal symbioses

Chromium immobilization by extra- and intraradical fungal structures of arbuscular mycorrhizal symbioses

The extent of mycorrhizal colonization of roots and its influence on plant growth and phosphorus content

Evidence that differences in phosphate metabolism in mycorrhizas formed by species of Glomus and Gigaspora might be related to their life‐cycle strategies

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