In planta production of a candidate vaccine against bovine papillomavirus type 1

Love, Andrew J.; Chapman, Sean; Matić, S.; Noris, Emanuela; Lomonossoff, George P.; Taliansky, Michael

doi:10.1007/s00425-012-1692-0

Cited by 50 publications

(32 citation statements)

References 44 publications

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“…The sequences were codon-optimized for the N. benthamiana genome to increase the yields of expressed protein [28]. The synthetic gene was used as template for insertion of a 6x His-tag at either the N- or C-terminus by the PCR DNA amplification method (see Methods).…”

Section: Resultsmentioning

confidence: 99%

Detection of Serum Antibodies to Hepatitis E Virus Based on HEV Genotype 3 ORF2 Capsid Protein Expressed in Nicotiana benthamiana

et al. 2017

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BackgroundHepatitis E virus (HEV) causes epidemics in developing countries and is primarily transmitted through the fecal-oral route. There have been recent reports on the zoonotic spread of the virus, and several animal species, primarily pigs, have been recognized as reservoirs of HEV. Because of its possible spread, there is an urgent need of a method for the cost-effective production of HEV proteins that can be used as diagnostic antigens for the serological detection of anti-HEV antibodies.MethodsThe HEV open reading frame (ORF)2 protein was purified from plant tissue by using immobilized metal-anion chromatography (IMAC). The recombinant protein was used to develop an in-house ELISA for testing anti-HEV antibodies in both human and swine sera. Thirty-six serum samples collected from patients with serologically proven HEV infection with commercial kits were tested for anti-HEV IgG antibodies by using the plant-expressed protein. Forty-five serum samples collected from apparently healthy pigs in Bulgarian farms were also tested.ResultsWe confirmed the transient expression and purification of a truncated version of the HEV genotype 3 capsid protein in Nicotiana benthamiana and its usefulness as a diagnostic antigen. ELISA showed the presence of anti-HEV IgG antibodies in 29 of the 36 human samples. The in-house ELISA showed anti-HEV IgG antibodies in 34 of the 45 pigs.ConclusionsWe describe a method for the production of HEV ORF2 protein in N. benthamiana and the usefulness of this protein for the serological detection of anti-HEV antibodies in both humans and swine.

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Section: Resultsmentioning

confidence: 99%

Detection of Serum Antibodies to Hepatitis E Virus Based on HEV Genotype 3 ORF2 Capsid Protein Expressed in Nicotiana benthamiana

et al. 2017

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“…The idea of developing a vaccine to combat BPV began with the infection of Shope papillomas extract in the 1940 decade (Shope, 1937). Since then, different vaccine models were proposed (Jarret et al , 1991; Gaukroger et al , 1996; Góes et al , 2008; Love et al , 2012; Mazzuchelli-de-Souza et al , 2013). However, none of them became a commercial product.…”

Section: Prophylactic and Therapeutic Vaccines Against Bpv And Hpvmentioning

confidence: 99%

Papillomaviruses: a systematic review

et al. 2017

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In the last decades, a group of viruses has received great attention due to its relationship with cancer development and its wide distribution throughout the vertebrates: the papillomaviruses. In this article, we aim to review some of the most relevant reports concerning the use of bovines as an experimental model for studies related to papillomaviruses. Moreover, the obtained data contributes to the development of strategies against the clinical consequences of bovine papillomaviruses (BPV) that have led to drastic hazards to the herds. To overcome the problem, the vaccines that we have been developing involve recombinant DNA technology, aiming at prophylactic and therapeutic procedures. It is important to point out that these strategies can be used as models for innovative procedures against HPV, as this virus is the main causal agent of cervical cancer, the second most fatal cancer in women.

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“…Although the CPMV- HT system has been used to generate VLPs from a number of other viruses, such as Hepatitis B core particles [17] and Human [27] and Bovine [28] Papillomavirus particles, all of which consist of a single type of subunit, the system was recently used to reconstruct fully assembled and immunogenic Bluetongue virus (BTV) VLPs [29 •• ]. This required not only the simultaneous expression of four structural proteins but was optimised by varying the expression levels between the proteins to favour the accumulation of fully assembled particles over assembly intermediates (Fig.…”

Section: Macromolecular Complexesmentioning

confidence: 99%