In planta transformation of rice (Oryza sativa) using thaumatin-like protein gene for enhancing resistance to sheath blight

Naseri, Gita

doi:10.5897/ajb11.3331

Cited by 15 publications

(8 citation statements)

References 47 publications

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“…In the presence of 100 lM acetosyringone, 32.6 % of Agrobacterium-infected setts were sprouted in 30 mg l -1 BASTA Ò solution, reaching a transformation efficiency of 11.6 % ( Table 2). A similar improvement of transformation with acetosyringone was reported in switchgrass, rice, Medicago truncatula, Arabidopsis, and cowpea (Chen et al 2010;Naseri et al 2012;Trieu et al 2000;Li et al 2009b;Adesoye et al 2010). Application of acetosyringone, above 100 lM reduced sett sprouting ability, which ultimately led to a lower transformation efficiency (Table 2).…”

Section: Influence Of Different Agrobacterium Strains and Acetosyringsupporting

confidence: 65%

Agrobacterium-mediated in planta genetic transformation of sugarcane setts

et al. 2015

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An efficient, reproducible, and genotype-independent in planta transformation has been developed for sugarcane using setts as explant. Traditional Agrobacterium-mediated genetic transformation and in vitro regeneration of sugarcane is a complex and time-consuming process. Development of an efficient Agrobacterium-mediated transformation protocol, which can produce a large number of transgenic plants in short duration is advantageous. Hence, in the present investigation, we developed a tissue culture-independent in planta genetic transformation system for sugarcane using setts collected from 6-month-old sugarcane plants. The sugarcane setts (nodal cuttings) were infected with three Agrobacterium tumefaciens strains harbouring pCAMBIA 1301-bar plasmid, and the transformants were selected against BASTA(®). Several parameters influencing the in planta transformation such as A. tumefaciens strains, acetosyringone, sonication and exposure to vacuum pressure, have been evaluated. The putatively transformed sugarcane plants were screened by GUS histochemical assay. Sugarcane setts were pricked and sonicated for 6 min and vacuum infiltered for 2 min at 500 mmHg in A. tumefaciens C58C1 suspension containing 100 µM acetosyringone, 0.1 % Silwett L-77 showed the highest transformation efficiency of 29.6 % (with var. Co 62175). The three-stage selection process completely eliminated the chimeric transgenic sugarcane plants. Among the five sugarcane varieties evaluated using the standardized protocol, var. Co 6907 showed the maximum transformation efficiency (32.6 %). The in planta transformation protocol described here is applicable to transfer the economically important genes into different varieties of sugarcane in relatively short time.

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Section: Influence Of Different Agrobacterium Strains and Acetosyringsupporting

confidence: 65%

Agrobacterium-mediated in planta genetic transformation of sugarcane setts

et al. 2015

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“…Keshamma et al (2008) also reported transformation frequency of 8.3% to 19% in cotton by employing the same method with a slight variation in colonization period as 60 min instead of 15 min used in the present investigation. Similar technique giving transformation frequencies of 24% and 9% were also reported in rice by Naseri et al (2012) and Allahi et al (2014) by the same injection method, but without colonization. Several other reports are in close conformity with these results viz.…”

Section: Recovery Of Putative Transgenics and Transformation Frequencysupporting

confidence: 70%

Optimization of genetic transformation method for an indica rice (Oryza sativa L.) variety Ratnagiri-711

Sawant¹,

Bhave²,

Sawardekar³

et al. 2020

IJGPB

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A study for method optimization of Agrobacterium-mediated genetic transformation for insect resistance was carried out for a rice variety Ratnagiri-711 showing better regenerability. Three different cry genes viz. cry1Aabc, cry1Fa1 and cry2Aa with a vector system consisting of the disarmed hyper virulent Agrobacterium tumefaciens strain EHA-105 harboring pBinAR or BinBt3 were used. The respective genes were linked to CaMV35S promoter and nptII gene under control of nos promoter and terminator. Scutellum-derived callus bits and embryonic shoot apical meristem of germinating seeds were used as target tissues for callus-mediated and in planta transformation, respectively. Kanamycin screening and PCR analysis was employed for confirmation of presence of transgene. Among five methods of colonization and co-cultivation tried with three cry genes, a callus-mediated transformation method consisting of 20 minutes colonization and 3 days co-cultivation with cry2Aa gene recorded highest transformation frequency (13.79%) but minimum survival (5.27%). On the contrary, considerable transformation frequency (6.35%) with highest survival (79.42%) was observed in an in planta method employing mild injury to embryonic shoot apical meristem of germinating seeds followed by injection of Agrobacterium having cry2Aa gene followed by 15 minutes colonization and then directly sowing in pots. Among three cry genes used, the gene cry2Aa was found most effective showing more transformation frequency.

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“…[76, 77], Colletotrichum gloeosporioides [78], Fusarium sp . [79], Podosphaera xanthii [57], Rhizoctonia solani [45, 80], Sclerotinia sclerotiorum [81], Verticillium dahliae [68] etc. The results of expression analysis of studied five cereals suggest the putative roles of TLPs in various abiotic and biotic stress conditions.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization revealed the role of thaumatin-like proteins in stress response in bread wheat

Sharma

Upadhyay

2020

Preprint

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BackgroundPR superfamily, pathogenesis related (PR) proteins divided into P1-PR17 subfamilies, is involved in the defense mechanism of plants. Thaumatin-like proteins (TLPs) are part of PR-5 family that facilitates resistance against abiotic and biotic stress responses.ResultsIn the present study, a total of 26, 27, 39, 93 and 37 TLPs with conserved signature-motif were identified in Brachypodium distachyon, Oryza sativa, Sorghum bicolor, Triticum aestivum and Zea mays, respectively. They were phylogenetically divided into 10 different clades. Majority of genes possessed one to three exons. Physicochemical analysis suggested significant differences between small and long TLPs. Majority consisted of signal peptide and localized in extracellular region. Evolutionary analyses suggested the role of duplication events in the expansion of this gene family. Tissue-specific expression of certain genes suggested their role in development, while differential expression revealed their putative roles in stress-response. The occurrence of assorted set of cis-regulatory elements further suggested their diverse functions. Co-expression analysis depicted their interaction with several stress responsive genes.ConclusionsThe detailed characterization of TLP proteins in cereals depicted their importance in combating various biotic and abiotic challenges. This study would be useful in further characterization of individual TLP genes in each plant, and various crop improvement programs in future studies.

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In planta transformation of rice (Oryza sativa) using thaumatin-like protein gene for enhancing resistance to sheath blight

Cited by 15 publications

References 47 publications

Agrobacterium-mediated in planta genetic transformation of sugarcane setts

Agrobacterium-mediated in planta genetic transformation of sugarcane setts

Optimization of genetic transformation method for an indica rice (Oryza sativa L.) variety Ratnagiri-711

Molecular characterization revealed the role of thaumatin-like proteins in stress response in bread wheat

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