Background: During the COVID-19 pandemic, Greek general surgeons faced a heavy workload and difficult decisions as they were redirected from surgical to COVID-related care. Similar to other pandemics in the past, Greek general surgeons reported experiencing low mood, increased stress, and anxiety, leading to psychological distress.Aim: This observational cross-sectional study aimed to investigate the stress levels and lifestyle of a sample of Greek general surgeons in relation to their contact with COVID-19.Methods: All general surgeons were invited to participate in a one-time self-administered online questionnaire, consisting of a 156-item self-reporting questionnaire. The data collected were about socio-demographic information, medical history, place of work, and years of work/experience. Six tools were used, and descriptive analysis was conducted for categorical and numerical variables.Results: Out of the 179 general surgeons who responded, 87.2% were male and 12.8% were female. The mean age for men was 51.47, and for women, it was 45.70. Men were more likely to be married with children compared to women.