In response to ‘A late complication following the insertion of hydrogel breast implants’

Cacou, Crina; Tinkler, Jeremy

doi:10.1016/j.bjps.2007.03.033

Cited by 2 publications

(1 citation statement)

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“…Hydrogels, a network of hydrophilic polymer chains, have been extensively used in various biomedical applications [8][9][10][11]. They have been synthesized with the ability to respond to temperature, pH, and the concentration of metabolites [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Modulation of cultured neural networks using neurotrophin release from hydrogel-coated microelectrode arrays

Jun¹,

Hynd

Dowell-Mesfin

et al. 2008

J. Neural Eng.

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Polyacrylamide and poly(ethylene glycol) diacrylate hydrogels were synthesized and characterized for use as drug release and substrates for neuron cell culture. Protein release kinetics was determined by incorporating bovine serum albumin (BSA) into hydrogels during polymerization. To determine if hydrogel incorporation and release affect bioactivity, alkaline phosphatase was incorporated into hydrogels and a released enzyme activity determined using the fluorescence-based ELF-97 assay. Hydrogels were then used to deliver a brain-derived neurotrophic factor (BDNF) from hydrogels polymerized over planar microelectrode arrays (MEAs). Primary hippocampal neurons were cultured on both control and neurotrophin-containing hydrogel-coated MEAs. The effect of released BDNF on neurite length and process arborization was investigated using automated image analysis. An increased spontaneous activity as a response to the released BDNF was recorded from the neurons cultured on the top of hydrogel layers. These results demonstrate that proteins of biological interest can be incorporated into hydrogels to modulate development and function of cultured neural networks. These results also set the stage for development of hydrogel-coated neural prosthetic devices for local delivery of various biologically active molecules.

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