In Saccharomyces cerevisiae, withdrawal of the carbon source results in detachment of glycolytic enzymes from the cytoskeleton and in actin reorganization

Espinoza-Simón, Emilio; Chiquete-Félix, Natalia; Morales-García, Lilia; Pedroza-Dávila, Ulrik; Pérez-Martı́nez, Xochitl; Araiza‐Olivera, Daniela; Torres-Quiroz, Francisco; Uribe‐Carvajal, Salvador

doi:10.1016/j.funbio.2019.10.005

Cited by 9 publications

(5 citation statements)

References 44 publications

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“…2C ), six of which were primarily composed of genes from the activation screens (I to VI). Cluster (I) contains genes encoding proteins in the inner membrane of mitochondria, including COX18 and PET122 , for which the null mutants are deficient in respiratory growth, 34 , 35 and TOM22 , which is central to protein import into mitochondria during the metabolic switch from fermentative to respiratory growth. 36 , 37 Cluster (II) connects via TLG2 , a gene encoding a t-SNARE protein involved in endosome to Golgi trafficking and the Cvt (cytoplasm-to-vacuole targeting) pathway 38 together with YPT52 that enables localization of the CORVET complex to endosomes.…”

Section: Resultsmentioning

confidence: 99%

Large scale microfluidic CRISPR screening for increased amylase secretion in yeast

Johansson,

Dulermo,

Jann

et al. 2023

Lab Chip

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Section: Resultsmentioning

confidence: 99%

Large scale microfluidic CRISPR screening for increased amylase secretion in yeast

Johansson,

Dulermo,

Jann

et al. 2023

Lab Chip

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“…This suggests that S. japonicus upper glycolysis is less tightly regulated by the flux through lower glycolysis, as compared to its sister species. Presumably, this feature allows S. japonicus to sustain rapid glycolysis in situations when it normally would be inhibited, such as low pH [74,75], or low environmental glucose levels [76][77][78]. The latter could be particularly important, since S. japonicus cannot rely on respiration as a metabolic strategy for dealing with starvation, unlike S. pombe [12,13,54].…”

Section: Discussionmentioning

confidence: 99%

Optimization of energy production and central carbon metabolism in a non-respiring eukaryote

Alam

Reichert

et al. 2022

Preprint

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Most eukaryotes respire oxygen, using it to generate biomass and energy. Yet, a few organisms lost the capacity to respire. Understanding how they manage biomass and energy production may illuminate the critical points at which respiration feeds into central carbon metabolism and explain possible routes to its optimization. Here we use two related fission yeasts, Schizosaccharomyces pombe and Schizosaccharomyces japonicus, as a comparative model system. We show that although S. japonicus does not respire oxygen, unlike S. pombe, it is capable of efficient NADH oxidation, amino acid synthesis and ATP generation. We probe possible optimization strategies using stable isotope tracing metabolomics, mass isotopologue distribution analysis, genetics, and physiological experiments. S. japonicus appears to have optimized cytosolic NADH oxidation via glycerol-3-phosphate synthesis. It runs a fully bifurcated TCA cycle, supporting higher amino acid production. Finally, it uses the pentose phosphate pathway both to support faster biomass generation and as a shunt to optimize glycolytic flux, thus producing more ATP than the respiro-fermenting S. pombe. By comparing two related organisms with vastly different metabolic strategies, our work highlights the versatility and plasticity of central carbon metabolism in eukaryotes, illuminating critical adaptations supporting the preferential use of glycolysis over oxidative phosphorylation.

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“…Correspondingly, pyruvate, a product related to glycolysis, was upregulated, which indicates that the glycolysis process was upregulated. Abnormal glycolysis aggravates the excessive consumption of carbon sources in a small area with an abnormal cytoskeleton, which might induce the detachment of glycolytic enzymes from the cytoskeleton [ 42 ]. Several enzymes related to glycolysis have been reported to be associated with the actin cytoskeleton [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics Integrated with Metabolomics Reveals 2-Methoxy-1, 4-Naphthoquinone-Based Carbon Dots Induced Molecular Shifts in Penicillium italicum

Chen

et al. 2022

JoF

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Penicillium italicum (P. italicum), a citrus blue mold, is a pathogenic fungus that greatly affects the postharvest quality of citrus fruits with significant economic loss. Our previous research showed that 2-methoxy-1, 4-naphthoquinone (MNQ) inhibited the growth of Penicillium italicum. However, the water dispersibility of MNQ will limit its further application. Herein, we synthesized MNQ-based carbon dots (2−CDs) with better water dispersibility, which showed a potential inhibitory effect on P. italicum (MIC = 2.8 μg/mL) better than that of MNQ (MIC = 5.0 μg/mL). Transcriptomics integrated with metabolomics reveals a total of 601 differentially enriched genes and 270 differentially accumulated metabolites that are co-mapped as disruptive activity on the cell cytoskeleton, glycolysis, and histone methylation. Furthermore, transmission electron microscopy analysis showed normal appearances and intracellular septum of P. italicum after treatment. These findings contribute tofurther understanding of the possible molecular action of 2−CDs.

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In Saccharomyces cerevisiae, withdrawal of the carbon source results in detachment of glycolytic enzymes from the cytoskeleton and in actin reorganization

Cited by 9 publications

References 44 publications

Large scale microfluidic CRISPR screening for increased amylase secretion in yeast

Large scale microfluidic CRISPR screening for increased amylase secretion in yeast

Optimization of energy production and central carbon metabolism in a non-respiring eukaryote

Transcriptomics Integrated with Metabolomics Reveals 2-Methoxy-1, 4-Naphthoquinone-Based Carbon Dots Induced Molecular Shifts in Penicillium italicum

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