In search of new stratification strategies: tissue proteomic profiling of papillary thyroid microcarcinoma in patients with localized disease and lateral neck metastases

Matovinovic, Filip; Novak, Rudjer; Hrkac, Stela; Salai, Grgur; Mocibob, Marko; Pranjic, Marija; Košec, Andro; Bedekovic, Vladimir; Grgurevic, Lovorka

doi:10.1007/s00432-023-05452-0

Cited by 5 publications

(1 citation statement)

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“…In the present study, the numerous correlations between miRNAs and between miRNAs and clinical parameters show the undoubted involvement of miRNAs in the clinical evolution of PTC, more precisely, a different and specific involvement of miRNAs in the evolution of certain clinical parameters in cPTC versus iFVPTC. Thus, miRNAs along with other molecules such as identified differentially expressed proteins could have a role as potential tissue biomarkers for PTC metastases [42].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Expression Profiling—Potential Molecular Discrimination of Papillary Thyroid Carcinoma Subtypes

Silaghi,

Pop,

Georgescu

et al. 2024

Biomedicines

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Recent research has revealed the importance of miRNAs in the diagnosis and clinical evolution of papillary thyroid cancer (PTC). We aim to identify a specific miRNA profile that could differentiate between specific subtypes of PTC. Methods: In this cross-sectional study, total RNA was extracted from paraffin-embedded tissues of 43 patients, 17 with an infiltrative follicular variant of PTC (iFVPTC) and 26 with a conventional variant of PTC (cPTC). Nine miRNAs were evaluated using qRT-PCR technology and specific miRNA assays. Results: We found specific patterns for cPTC and iFVPTC, such as miRNA altered in both types of tumours (miR-146b-5p, miR-181a-5p, miR-221-3p, miR-21-5p and miR-222-3p) and two miRNAs significantly expressed only in cPTC (miR-20b-5p, miR-21-5p). The iFVPTC group presented strong and moderate correlations between miRNA expression and clinical data. miR-221-3p, miR-195-5p, miR-181-5p, miR-146b-5p and miR-222 were correlated with age, tumour size (TS) or lymph node metastases (N), while only miR-20b-5p, miR-195-5p and miR-181-5p were correlated with TS, N and age in the cPTC group. Conclusions: The present study allowed the identification of a signature of two miRNAs to confirm miRNA differences between the two histological subtypes of TC. Our results provide advances in the molecular diagnosis of TC and could help to improve the diagnostic performance of already existing molecular classifiers.

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Section: Discussionmentioning

confidence: 99%