The evaluation of physical fitness in team sports is enjoying greater importance in the training of professional teams. The objectives of this research were to characterize physical fitness and game indicators based on the game position. This is an empirical study, with a quantitative, descriptive and cross-sectional methodology. In addition, different relationships between the level of physical fitness and the game indicators during the competition were determined. Finally, a predictive analysis of the selected variables was carried out in order to know the importance of the variables in the performance and at what time of the season they had the greatest impact. For this, a professional female basketball team (n = 12) with a mean age of 25.25 ± 7.617 years, height 178.25 ± 9.206 cm and a body mass of 72.33 ± 11.657 kg was analyzed. Each player was equipped with a WIMUPRO inertial device, and all competition statistics were analyzed. The results obtained show that there were no differences among all the skills evaluated and game indicators depending on the game position. Likewise, a relationship was determined between the level of physical fitness and the technical–tactical contribution, being different between different times of the season. Moreover, physical fitness only predicted the player’s final performance in the competition for female player centers in the first and second rounds of the championship, and for forwards in the first round. Four physical-physiological profiles with contributions to the different ones during the competition were also determined. Finally, three groups of female players by playing position were identified according to their sport performance, namely PIR, high, medium and low ratings, associated with physical performances. In all groups, there were significant differences between playing positions, PIR and physical performances.