In silico ADMET analysis, Molecular docking and in vivo anti diabetic activity of polyherbal tea bag formulation in Streptozotocin-nicotinamide induced diabetic rats

Quazi, Aamir; Mohsina, F. P; P., Faheem I.; Priya, S.

doi:10.53730/ijhs.v6ns3.5189

Cited by 10 publications

(6 citation statements)

References 35 publications

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“…Antioxidant activity of polyherbal formulation has been calculated by the DPPH radical scavenging assay [ 24 ], the GTA (green tea antioxidants) activity [ 25 ], and by using the thiobarbituric acid reactive substance assay (TBARS) [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Assessment of Antioxidative and Alpha-Amylase Potential of Polyherbal Extract

Patwekar

Mezni

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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The present study aims to prepare a polyherbal formulation (PHF) of Azadirachta indica (Neem), Aloe barbadensis (Aloe vera), Allium sativum (garlic), Acacia arabica (Babul), and Aegle marmelos (Bel) and evaluation of antidiabetic and antioxidant activity utilizing the in vitro model. Air-dried powder of 5 medicinal plants, which are divided into equal portions, and PHF, is prepared by the soxhlet technique using polar and nonpolar solvents. The PHF is screened for the phytochemical screening, and then the antidiabetic activity is determined by alpha-amylase inhibition. The extracts thus obtained are also subjected to the inhibition assay by the use of (DNS) dinitro salicylic acid. The antioxidant activity was determined by the DPPH radical scavenging assay, H2O2 scavenging assay, and TBARS assay. In in vitro study, the result revealed polyherbal formulation in which hot water extract has the topmost inhibitory effect on alpha-amylase activity, ranging from 20.4% to 79.5% with an IC50 value of 48.98 ± 0.31 μg/ml. This extract clearly showed the effective lowering of postprandial hypertriglyceridemia (PPHG). In the antioxidant activity carried out by using the (DPPH) radical scavenging assay, the highest result was obtained by the concentration of 250 μg/ml, which was around 77.2 ± 0.05 with statistical significance compared with control (a: p < 0.01 ; b: p < 0.001 ), while in the GTA method, the highest result was obtained by the concentration of 250 μg/ml, which was around 78.2 ± 0.05, and in the case of the TBARS assay, the concentration of 250 μg/ml gave around 76.2 ± 0.03 anti-oxidant value. In conclusion, the study shows that polyherbal formulation has superior antidiabetic activity and antioxidant properties.

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Section: Methodsmentioning

confidence: 99%

Assessment of Antioxidative and Alpha-Amylase Potential of Polyherbal Extract

Patwekar

Mezni

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…JTD granules were composed of Panax ginseng C.A. Meyer 9 g, Gastrodia elata 9 g, Atractylodes macrocephala 10 g, Morindae Officinalis Radix 6 g, Acorus tatarinowii Schott 6 g, Rhizoma coptidis 3 g, and Semen Cuscutae 12 g. The JTD granules are prepared in accordance with the previous methods [ 32 , 33 ]. The collected CHM was washed with water to remove any dust or foreign particles present on them and shade-dried for one week at room temperature to avoid excessive loss of volatile components.…”

Section: Methodsmentioning

confidence: 99%

Integrated Network Pharmacology and Proteomic Analyses of Targets and Mechanisms of Jianpi Tianjing Decoction in Treating Vascular Dementia

Liu

Gong

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Background. Vascular dementia (VD), associated with cerebrovascular injury, is characterized by severe cognitive impairment. Jianpi Tianjing Decoction (JTD) has been widely used to treat VD. However, its molecular targets and mechanisms of action in this treatment remain unclear. This study integrated network pharmacology and proteomics to identify targets and mechanisms of JTD in the treatment of VD and to provide new insights and goals for clinical treatments. Methods. Systematic network pharmacology was used to identify active chemical compositions, potential targets, and mechanisms of JTD in VD treatment. Then, a mouse model of VD was induced via transient bilateral common carotid artery occlusion to verify the identified targets and mechanisms of JTD against VD using 4D label-free quantitative proteomics. Results. By screening active chemical compositions and potential targets in relevant databases, 187 active chemical compositions and 416 disease-related compound targets were identified. In vivo experiments showed that JTD improved learning and memory in mice. Proteomics also identified 112 differentially expressed proteins in the model and sham groups and the JTD and model groups. Integrating the network pharmacology and proteomics results revealed that JTD may regulate expressions of cytochrome c oxidase subunit 7C, metabotropic glutamate receptor 2, Slc30a1 zinc transporter 1, and apolipoprotein A-IV in VD mice and that their mechanisms involve biological processes like oxidative phosphorylation, regulation of neuron death, glutamate secretion, cellular ion homeostasis, and lipoprotein metabolism. Conclusions. JTD may suppress VD development via multiple components, targets, and pathways. It may thus serve as a complementary treatment option for patients with VD.

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“…XFC is prepared from natural medicines such as T. wilfordii, and the research and development of natural medicinal plants have been a research hotspot in this direction [29]. Molecular docking is commonly employed to simulate the binding pattern and affinity between small molecular compounds contained in natural drugs and targets [30]. The results suggested that the structure of triptolide and quercetin was stable with the key target, indicating that triptolide and quercetin may be the key active components of XFC.…”

Section: Effects Of Xfc On Joint Symptoms Inflammatory Factors and Tl...mentioning

confidence: 99%

Exploration of the molecular mechanism guiding Xinfeng capsule regulatory mechanism for rheumatoid arthritis inflammation

Zhu

2024

Am J Transl Res

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Objectives: Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation of the joint synovium. The traditional Chinese medicine Xinfeng capsule (XFC) has a remarkable alleviating effect on inflammatory symptoms, such as joint pain and swelling, in patients with RA. However, the underlying mechanism of action remains to be elucidated. This study intended to conduct network pharmacology, animal experiments, data mining, and molecular docking to explore the molecular mechanism through which XFC can improve the inflammatory symptoms of RA. Methods: The Apriori association rules and a random walk model were employed to evaluate the effect of XFC on the clinical inflammatory indexes of RA. The active ingredients and the potential target genes of XFC were obtained from public databases. Based on the search tool for recurring instances of neighboring genes (STRING) database, the Database for Annotation, Visualization and Integrated Discovery (DAVID) database, Cytoscape software, and molecular docking method, the molecular mechanism by which XFC acts on RA was also analyzed. Finally, an adjuvant arthritis rat model was established to verify the effects of XFC on inflammationrelated signaling pathways and inflammatory factors. Results: XFC significantly reduced the level of C-reactive protein (CRP), vascular endothelial growth factor (VEGF), and the erythrocyte sedimentation rate (ESR). The docking space structures of the active ingredients in XFC, namely triptolide and quercetin, and the key targets were stable. Inflammation-related biological processes were identified as the key factors involved in the development of RA, and the regulation of the toll-like receptor (TLR) signaling pathway may be the key link for XFC toward improving the inflammatory state of RA. The expression levels of toll-like receptor 4 (TLR4), myeloid differentiation primary response protein MyD88 (MyD88), interleukin-1 receptor-associated kinase 1 (IRAK1), TNF receptor-associated factor 6 (TRAF6), TGF-beta-activated kinase 1 (TAK1), phospho-Inhibitor of NF-κB kinaseβ (p-IKKβ), phospho-Nuclear factor-k-gene binding (p-NF-κB), and interleukin-1β (IL-1β) can all be decreased by XFC. XFC improves joint inflammation symptoms by lowering pro-inflammatory factors tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interferon-γ (INF-γ) levels. Conclusions: XFC could effectively improve the clinical inflammatory indexes of RA. The active ingredients of XFC improved the inflammatory state of RA by regulating the TLR-signaling pathway.

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In silico ADMET analysis, Molecular docking and in vivo anti diabetic activity of polyherbal tea bag formulation in Streptozotocin-nicotinamide induced diabetic rats

Cited by 10 publications

References 35 publications

Assessment of Antioxidative and Alpha-Amylase Potential of Polyherbal Extract

Assessment of Antioxidative and Alpha-Amylase Potential of Polyherbal Extract

Integrated Network Pharmacology and Proteomic Analyses of Targets and Mechanisms of Jianpi Tianjing Decoction in Treating Vascular Dementia

Exploration of the molecular mechanism guiding Xinfeng capsule regulatory mechanism for rheumatoid arthritis inflammation

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