In silico analysis of genomic variables associated to HPV16 integration sites

Diaz-Moreno, Nicole; Osorio, Julio; García‐Perdomo, Herney Andrés; Castillo, Andrés

doi:10.22354/in.v24i2.836

Cited by 1 publication

(1 citation statement)

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“…showed that USP4 is downregulated in HPV-negative oropharyngeal cancer (Lohavanichbutr et al 2009 ). Furthermore, the USP4 gene is an integration site for HPV16 in cervical and oropharyngeal squamous cell carcinoma, which may lead to alteration of gene expression (Díaz-Moreno et al 2020 ). Hou et al showed that USP4 has a tumor suppressor role in HNSCC in vitro (Hou et al 2013 ).…”

Section: Discussionmentioning

confidence: 99%

High USP4 mRNA is associated with an HPV-positive status in head and neck squamous cell carcinoma patients

Al-Gboore

Jank

Brkic

et al. 2023

J Cancer Res Clin Oncol

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Introduction Head and neck squamous cell carcinoma (HNSCC) is among the most common cancers in the world with a low survival rate and common diagnosis at late stages. Deubiquitination of proteins is involved in tumor growth, metastasis, apoptosis, and immunosuppressive pathways. The impact of the ubiquitin-specific protease (USP4) on survival was only scarcely investigated so far. The goal of our research was to analyze the association of USP4 expression with prognosis and clinicopathological features in HNSCC. Methods USP4 mRNA levels were derived from The Cancer Genome Atlas (TCGA) for a cohort of 510 patients. Protein expression of USP4 was analyzed by immunohistochemistry in a second cohort of 113 patients. Associations between USP4 levels and overall survival, disease-free survival and clinicopathological data were analyzed. Results High levels of USP4 mRNA were associated with prolonged overall survival in univariable analysis. There was no more association with survival after correction for the confounders HPV, stage and smoker status. High USP4 mRNA levels were linked to a lower T-stage, the patient’s age at diagnosis, and a positive HPV status. USP4 protein levels were not associated with prognosis or other features. Conclusion Since high USP4 mRNA was not an independent prognostic marker, we assume that the association is a result of the correlation of high USP4 mRNA with an HPV-positive status. Therefore, further investigation of USP4 mRNA and its association with the HPV status of HNSCC patients is warranted.

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Section: Discussionmentioning

confidence: 99%