In silico and in situ characterization of the zebrafish (Danio rerio) gnrh3 (sGnRH) gene

Torgersen, Jacob; Nourizadeh-Lillabadi, Rasoul; Husebye, Harald; Aleström, Peter

doi:10.1186/1471-2164-3-25

Cited by 44 publications

(7 citation statements)

References 78 publications

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“…Previous studies have shown that the increase of cGnRH signaling promotes animals’ survival functions, such as food intake, whereas the modulation of sGnRH signaling transduction directly affects animals’ sexual activity and reproduction. In zebrafish, the olfactory TNs synthesize and release sGnRH [ 19 ]. Previously, we demonstrated that through the olfacto-visual centrifugal pathway, activation of the olfactory neurons decreases the release of dopamine from retinal DA-IPCs.…”

Section: Discussionmentioning

confidence: 99%

GnRH-mediated olfactory and visual inputs promote mating-like behaviors in male zebrafish

Wojtowicz

Malin

et al. 2017

PLoS ONE

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The engagement of sexual behaviors is regulated by a number of factors which include gene expression, hormone circulation, and multi-sensory information integration. In zebrafish, when a male and a female are placed in the same container, they show mating-like behaviors regardless of whether they are kept together or separated by a net. No mating-like behaviors are observed when same-sex animals are put together. Through the olfacto-visual centrifugal pathway, activation of the terminalis nerve in the olfactory bulb increases GnRH signaling in the brain and triggers mating-like behaviors between males. In zebrafish mutants or wild-type fish in which the olfacto-visual centrifugal pathway is impaired or chemically ablated, in response to odor stimulation the mating-like behaviors between males are no longer evident. Together, the data suggest that the combination of olfactory and visual signals alter male zebrafish's mating-like behaviors via GnRH signaling.

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Section: Discussionmentioning

confidence: 99%

GnRH-mediated olfactory and visual inputs promote mating-like behaviors in male zebrafish

Wojtowicz

Malin

et al. 2017

PLoS ONE

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“…In the literature, the immunocytochemical localization of GnRH in the hypothalamus of the adult zebrafish has been inconsistent, with varied expression patterns reported including the suggestion that the zebrafish have no GnRH positive cell bodies in the POA. In addition to immunocytochemical studies, reports using GFP reporter lines to visualize GnRH cells in vivo ( Torgersen et al, 2002 ; Abraham et al, 2009 , 2008 ; Ramakrishnan et al, 2010 ; Zhao et al, 2013 ) have also been inconsistent and do not agree with endogenous gene expression data ( Gopinath et al, 2004 ; Steven et al, 2003 ). Using three different antibodies that detect GnRH, we have been able to unequivocally localize GnRH containing cell bodies in the parvocellular nucleus of the adult hypothalamus.…”

Section: Discussionmentioning

confidence: 99%

Zebrafish adult-derived hypothalamic neurospheres generate gonadotropin-releasing hormone (GnRH) neurons

et al. 2015

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Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide essential for fertility in vertebrates. Human male patients lacking GnRH and treated with hormone therapy can remain fertile after cessation of treatment suggesting that new GnRH neurons can be generated during adult life. We used zebrafish to investigate the neurogenic potential of the adult hypothalamus. Previously we have characterized the development of GnRH cells in the zebrafish linking genetic pathways to the differentiation of neuromodulatory and endocrine GnRH cells in specific regions of the brain. Here, we developed a new method to obtain neural progenitors from the adult hypothalamus in vitro. Using this system, we show that neurospheres derived from the adult hypothalamus can be maintained in culture and subsequently differentiate glia and neurons. Importantly, the adult derived progenitors differentiate into neurons containing GnRH and the number of cells is increased through exposure to either testosterone or GnRH, hormones used in therapeutic treatment in humans. Finally, we show in vivo that a neurogenic niche in the hypothalamus contains GnRH positive neurons. Thus, we demonstrated for the first time that neurospheres can be derived from the hypothalamus of the adult zebrafish and that these neural progenitors are capable of producing GnRH containing neurons.

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“…The present study indicates the anorexigenic action of GnRH2 in the zebrafish, as is the case in female musk shrew and goldfish ( Hoskins et al, 2008 ; Matsuda et al, 2008 ; Kang et al, 2011 ), indicating that it may be involved generally in the regulation of feeding behavior in vertebrates. GnRH3 is widely distributed in several regions of the zebrafish brain, including the olfactory bulb, the area of the terminal nerve, and telencephalon, and GnRH3 is implicated in pituitary control ( Torgersen et al, 2002 ; Steven et al, 2003 ; Palevitch et al, 2007 ). On the other hand, GnRH2-expressing neurons are localized mainly in the midbrain tegmentum ( Palevitch et al, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Gonadotropin-releasing hormone 2 suppresses food intake in the zebrafish, Danio rerio

Nishiguchi¹,

Azuma²,

Yokobori³

et al. 2012

Front. Endocrin.

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Gonadotropin-releasing hormone (GnRH) is an evolutionarily conserved neuropeptide with 10 amino acid residues, of which several structural variants exist. A molecular form known as GnRH2 ([His5 Trp7 Tyr8]GnRH, also known as chicken GnRH II) is widely distributed in vertebrates except for rodents, and has recently been implicated in the regulation of feeding behavior in goldfish. However, the influence of GnRH2 on feeding behavior in other fish has not yet been studied. In the present study, therefore, we investigated the role of GnRH2 in the regulation of feeding behavior in a zebrafish model, and examined its involvement in food intake after intracerebroventricular (ICV) administration. ICV injection of GnRH2 at 0.1 and 1 pmol/g body weight (BW) induced a marked decrease of food consumption in a dose-dependent manner during 30 min after feeding. Cumulative food intake was significantly decreased by ICV injection of GnRH2 at 1 pmol/g BW during the 30-min post-treatment observation period. The anorexigenic action of GnRH2 was completely blocked by treatment with the GnRH type I receptor antagonist Antide at 25 pmol/g BW. We also examined the effect of feeding condition on the expression level of the GnRH2 transcript in the hypothalamus. Levels of GnRH2 mRNA obtained from fish that had been provided excess food for 7 days were higher than those in fish that had been fed normally. These results suggest that, in zebrafish, GnRH2 acts as an anorexigenic factor, as is the case in goldfish.

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In silico and in situ characterization of the zebrafish (Danio rerio) gnrh3 (sGnRH) gene

Cited by 44 publications

References 78 publications

GnRH-mediated olfactory and visual inputs promote mating-like behaviors in male zebrafish

GnRH-mediated olfactory and visual inputs promote mating-like behaviors in male zebrafish

Zebrafish adult-derived hypothalamic neurospheres generate gonadotropin-releasing hormone (GnRH) neurons

Gonadotropin-releasing hormone 2 suppresses food intake in the zebrafish, Danio rerio

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