In Silico and In Vitro Analyses Identified Three Amino Acid Residues Critical to the Catalysis of Two Aphid Farnesyl Diphosphate Synthase

Sun, Xiaohong; Li, Zhengxi

doi:10.1007/s10930-012-9421-x

Cited by 11 publications

(10 citation statements)

References 28 publications

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“…Interestingly, only one FPPS gene existed in the cotton aphid Aphis gossypii , whereas most other aphid species (eg M . persicae and Rhopalosiphum padi ) had two FPPS genes (Zhang and Li, ; Ma et al, ; Sun and Li, ). The GGPP synthase (GGPPS) was functionally analysed in vitro in A .…”

Section: Introductionmentioning

confidence: 99%

“…The FPP synthases (FPPSs) displayed dual GPP synthase (GPPS)/FPPS activity in the green peach aphid Myzus persicae (Lewis et al, 2008;Vandermoten et al, 2008;Zhang and Li, 2012). Interestingly, only one FPPS gene existed in the cotton aphid Aphis gossypii, whereas most other aphid species (eg M. persicae and Rhopalosiphum padi) had two FPPS genes (Zhang and Li, 2008;Ma et al, 2010;Sun and Li, 2012). The GGPP synthase (GGPPS) First published online 12 September 2018.…”

Section: Introductionmentioning

confidence: 99%

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The terpenoid backbone biosynthesis pathway directly affects the biosynthesis of alarm pheromone in the aphid

Sun

2018

Insect Molecular Biology

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The terpenoid backbone biosynthesis pathway is responsible for the synthesis of different backbones for terpenoids; (E)-β-farnesene (EβF), a sesquiterpene, is the major component of aphid alarm pheromone. Our previous studies eliminated the possibility of host plants and endosymbionts as the sources of EβF, and we thus speculate that the terpenoid pathway might affect the biosynthesis of EβF in aphids. First, the transcriptional responses of four genes encoding farnesyl diphosphate synthase (FPPS), geranylgeranyl diphosphate synthase (GGPPS) and decaprenyl diphosphate synthase in the cotton aphid Aphis gossypii to simulated stimulation were analysed using quantitative real-time PCR, showing an immediate decrease in the transcript abundances of the four genes. Next, RNA-interference-mediated gene knockdown was performed, indicating that fpps knockdown caused a significant cost in terms of body size and fecundity. Finally, an association analysis of gene knockdown with the amount of EβF was conducted, revealing that the concentration of EβF per milligram of aphid was drastically decreased in response to fpps knockdown, whereas ggpps knockdown significantly raised the concentration of EβF. Our data support a peculiar mode of biosynthesis and storage of the aphid alarm pheromone that relies directly on the terpenoid backbone biosynthesis pathway in the aphid.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The terpenoid backbone biosynthesis pathway directly affects the biosynthesis of alarm pheromone in the aphid

Sun

2018

Insect Molecular Biology

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“…In our laboratory, we cloned two full-length cDNAs ( RpFPPS1 and RpFPPS2 ) coding for FPPS in the bird cherry-oat aphid Rhopalosiphum padi (Sun & Li, 2012). Recombinant expression and in vitro enzymatic activity assay showed that both FPPSs could utilize isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) to produce FPP.…”

Section: Introductionmentioning

confidence: 99%

Production of alarm pheromone starts at embryo stage and is modulated by rearing conditions and farnesyl diphosphate synthase genes in the bird cherry-oat aphid Rhopalosiphum padi

Sun

2019

Bull. Entomol. Res.

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The major component of aphid alarm pheromone is (E)-β-farnesene (EβF), but the molecular mechanisms of EβF synthesis are poorly understood. Here we established a biological model to study the modulation of EβF synthesis in the bird cherry-oat aphid Rhopalosiphum padi by using quantitative polymerase chain reaction, gas chromatography/mass spectrometry and RNA interference. Our results showed that the rearing conditions significantly affected the weight of adult and modulated EβF synthesis in a transgenerational manner. Specifically, the quantity of EβF per milligram of aphid was significantly reduced in the individually reared adult or 1st-instar nymphs derived from 1-day-old adult reared individually, but EβF in the nymph derived from 2-day-old adult that experienced collective conditions returned to normal. Further study revealed that the production of EβF started in embryo and was extended to early nymphal stage, which was modulated by farnesyl diphosphate synthase genes (RpFPPS1 and RpFPPS2) and rearing conditions. Knockdown of RpFPPS1 and RpFPPS2 confirmed the role played by FPPS in the biosynthesis of aphid alarm pheromone. Our results suggested that the production of EβF starts at the embryo stage and is modulated by FPPS and rearing conditions in R. padi, which sheds lights on the modulatory mechanisms of EβF in the aphid.

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“…In recent years, FPPS genes have been studied in aphids partially due to their association with the biosynthesis of EβF (Vandermoten et al, ). In our group, we have isolated and characterized several FPPSs from different aphid species, such as the green peach aphid Myzus persicae (Y. L. Zhang & Li, , 2012), the cotton aphid Aphis gossypii (Ma, Sun, Zhang, Li, & Shen, ) and R. padi (X. F. Sun & Li, ). Moreover, we cloned all isoprenyl diphosphate synthases in the terpenoid backbone biosynthesis pathway of A. gossypii , including FPPS, geranylgeranyl diphosphate synthase (GGPPS) and decaprenyl diphosphate synthase (DPPS; H. Zhang & Li, , ).…”

Section: Introductionmentioning

confidence: 99%

Both farnesyl diphosphate synthase genes are involved in the production of alarm pheromone in the green peach aphid Myzus persicae

Cheng

2018

Arch Insect Biochem Physiol

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Farnesyl diphosphate synthase (FPPS) catalyzes the formation of FPP, providing the precursor for the biosynthesis of (E)-βfarnesene (EβF) in plants, but it is unknown if FPPS supplies the precursor for the biosynthesis of EβF, the major component of aphid alarm pheromone, though our previous studies support the hypothesis that EβF is synthesized by the aphid itself. Here, we used two cohorts of the green peach aphid Myzus persicae separately, reared on pepper plant and artificial diet to test the correlations among droplet emission, EβF quantity, and FPPS gene expression. It was found that the proportion of aphids emitting cornicle droplets and the quantity of EβF per milligram of aphid were both significantly different between the two cohorts, which were positively correlated with the expression of the two FPPS genes (MpFPPS1/2) in M. persicae. These results were further confirmed by RNAi-mediated knockdown of MpFPPS1/2. Specifically, knockdown of MpFPPS1/2 imposed no significant cost on the survival of aphid but remarkably increased the number of offspring per aphid; most importantly, knockdown of MpFPPS1/2 significantly reduced the proportion of aphids emitting droplets and the quantity of EβF calculated as per the weight of aphid. Our results suggest that both FPPS genes are involved in the production of EβF in M. persicae and cornicle droplet emission is closely associated with the EβF release in the aphid. K E Y W O R D S (E)-β-farnesene, farnesyl diphosphate synthase, Myzus persicae, RNAi Arch. Insect Biochem. Physiol. 2019;100:e21530.wileyonlinelibrary.com/journal/arch

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In Silico and In Vitro Analyses Identified Three Amino Acid Residues Critical to the Catalysis of Two Aphid Farnesyl Diphosphate Synthase

Cited by 11 publications

References 28 publications

The terpenoid backbone biosynthesis pathway directly affects the biosynthesis of alarm pheromone in the aphid

The terpenoid backbone biosynthesis pathway directly affects the biosynthesis of alarm pheromone in the aphid

Production of alarm pheromone starts at embryo stage and is modulated by rearing conditions and farnesyl diphosphate synthase genes in the bird cherry-oat aphid Rhopalosiphum padi

Both farnesyl diphosphate synthase genes are involved in the production of alarm pheromone in the green peach aphid Myzus persicae

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