In silico and in vitro approaches allow the identification of the Prosystemin molecular network

Natale, Roberto; Coppola, Mariangela; D’Agostino, Nunzio; Zhang, Youjun; Fernie, Alisdair R.; Castaldi, Valeria; Rao, Rosa

doi:10.1016/j.csbj.2022.12.006

Cited by 2 publications

(2 citation statements)

References 114 publications

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“…Similar induction was observed in plant treated with protein fragments (Fig. 5 This group includes tomato ProSys, that is involved in the release of the Sys peptide and serves as a key hub for multiple plant stress-related activities (Natale et al, 2023). Indeed, its interaction plasticity, favoured by its disordered feature , makes ProSys to cope with plant response to both biotic and abiotic stresses.…”

Section: Gene Expressions Analysissupporting

confidence: 60%

Prosystemin N-terminal protein fragments mitigate salt stress damages in tomato plants

Criscuolo,

Castaldi,

Aprile

et al. 2024

Preprint

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Understanding and exploiting the intrinsic mechanisms of tolerance to multiple stresses in plants is the new frontier of sustainable agriculture, since environmental challenges often occur simultaneously in agricultural systems. We recently identified three fragments, named PS1-70, PS1-120 and G, in the scaffold of prosystemin, the protein precursor of tomato systemin. These protein fragments efficiently protect tomato plants against Botrytis cinerea and Spodoptera littoralis larvae attacks by inducing defence-related genes. Since it was previously demonstrated that prosystemin protects tomato plants also against soil salinity, we analyzed the ability of PS1-70, PS1-120 and G to confer salt tolerance. As expected, the application of 150 mM NaCl induced 24% reduction of shoot fresh weight. The treatment with PS1-70 and G induced 9% and 8% increase of shoot fresh weight. In addition, under salt stress, there is a significant increase in root biomass in treated plants suggesting that the treatment mitigated salt stress. Noteworthy, fragments application improved the growth of shoots, indicating a biostimulant activity on tomato growth. These data correlated with the upregulation of key stress-related genes, (CAT2, APX2, and HSP90), associated with the activation of antioxidant and free radical scavenging reactions in stressed plant cells. Our results add novel tools to the complex problem of sustainable crop protection against different environmental stresses.

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Section: Gene Expressions Analysissupporting

confidence: 60%

Prosystemin N-terminal protein fragments mitigate salt stress damages in tomato plants

Criscuolo,

Castaldi,

Aprile

et al. 2024

Preprint

Self Cite

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“…In the present study sugar metabolism related protein such as NAD‐dependent epimerase/dehydratase, trifunctional UDP‐glucose 4, 6‐dehydratase and UDP‐glucuronic acid decarboxylase 6 were up regulated. To reduce oxidative damage, NAD‐dependent epimerase/dehydratase enzyme employs nucleotide sugar substrates in a range of chemical processes, including those of the ROS scavenging system (Natale et al, 2023). The upregulated expression of UDP‐sulfoquinovose synthase in response to P deficiency implies a potential substitution of specific membrane phospholipids by sulfoquinovosyl diacylglycerol (SQDG) (Zhang et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Analysis of physiological response and differential protein expression of Paramichelia baillonii saplings under phosphorus deficiency

Ali,

Wu,

Chen

et al. 2024

Physiologia Plantarum

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Paramichelia baillonii is a rare and fast‐growing tree species in subtropical China. The acidic red soil in southern China severely limits its growth as it lacks sufficient available phosphorus (P), resulting in declining soil fertility and nutrient availability. The effect of P deficiency on P. Baillonii growth, root attributes, and physiological response has not yet been reported. Understanding the adaptability of P. baillonii to low‐P conditions can improve afforestation and soil management in southern China. Therefore, we conducted a pot experiment on 2‐year‐old saplings and treated them with different P levels. Results showed that P deficiency (0–5 mg L−1) decreased growth attributes, root morphological traits, and nutrient uptake of P. baillonii saplings compared to control (CK). Similarly, reduction in chlorophyll a, b, total chlorophyll, net photosynthetic rate (Pn), transpiration rate (Tr), and Gs were seen in low P treatment saplings compared to CK. Whereas superoxide dismutase, peroxidase, malondialdehyde, acid phosphatase activity, and soluble protein content increased with increasing P‐deficiency up to 5 mg L−1, and soluble sugar showed oppsite trend. Moreover, the proteomics analysis identified 2721 proteins, 196 showing differential expression, with 90 up‐ and 106 down‐regulated. Importantly, the metabolic activities increased in the pentose phosphate pathway, starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism, and phenylpropanoid biosynthesis pathways to sustain regular plant growth under P deficiency. This study delves into the dynamic morpho‐physiological and proteomic changes in response to P deficiency. Overall, growth and nutrient uptake were reduced, countered by adaptive biochemical and proteomic shifts, including heightened antioxidant activities and modifications in metabolic pathways, highlighting the resilient strategies of P. baillonii saplings under P deficiency.

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In silico and in vitro approaches allow the identification of the Prosystemin molecular network

Cited by 2 publications

References 114 publications

Prosystemin N-terminal protein fragments mitigate salt stress damages in tomato plants

Prosystemin N-terminal protein fragments mitigate salt stress damages in tomato plants

Analysis of physiological response and differential protein expression of Paramichelia baillonii saplings under phosphorus deficiency

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