In silico and in vitro Assessment of Indigenous Organic Practices on Germination and Seedling Metrics of Compact Cotton using GerminaR R Software Package

Abstract

Background: Cotton crop is sensitive in its young stages i.e. stand establishment. Cotton seeds are fuzzy and messy in handling and field sowing. Delinting is a necessary process done commonly using acids and are comprehensively not protective. Optimal plant population, growth and persistence are strongly influenced by germination and establishment. In line with this, it is necessary and needful of an hour to find an alternative, eco-friendly, ecologically safe and low cost seed treatment method. Methods: The experiment was conducted in two methods viz., a petridish method and roll-towel method with seed treatment methods viz., cowdung and red earth mix treated with and without biofertilizer and compared with acid-delinted and fuzzy seeds. The germino-metrics were determined using GerminaR package, an interactive web application and statistical analysis was performed using R software package v. 4.2.1. Result: The summative effect of seed treatment with fresh cow dung, red earth and biofertilizer (Azospirillum and Phosphobacteria) per kg of seeds at respective quantities (0.5:0.25:0.12) recorded significantly maximal germination indices and seedling growth determinants. This present study would be a complementary to comprehensively promote the use of indigenously available natural materials as an alternative to acids in cotton seed delinting.