In silico characterization and expression analysis of the multigene family encoding the Bowman–Birk protease inhibitor in soybean

Abstract

The Bowman-Birk (BBI) protease inhibitors can be used as source of sulfur amino acids, can regulate endogenous protease activity during seed germination and during the defense response of plants to pathogens. In soybean this family has not been fully described. The goal of this work was to characterize in silico and analyze the expression of the members of this family in soybean. We identified 11 potential BBI genes in the soybean genome. In each one of them at least a characteristic BBI conserved domain was detected in addition to a potential signal peptide. The sequences have been positioned in the soybean physical map and the promoter regions were analyzed with respect to known regulatory elements. Elements related to seed-specific expression and also to response to biotic and abiotic stresses have been identified. Based on the in silico analysis and also on quantitative RT-PCR data it was concluded that BBI-A, BBI-CII and BBI-DII are expressed specifically in the seed. The expression profiles of these three genes are similar along seed development. Their expressions reach a maximum in the intermediate stages and decrease as the seed matures. The BBI-DII transcripts are the most abundant ones followed by those of BBI-A and BBI-CII.

Transcript profiling of serine- and cysteine protease inhibitors inTriticum aestivumvarieties with different drought tolerance

“…Bowman-Birk PIs (BBPIs) are canonical inhibitors of serine protease of the trypsin and chymotrypsin type (de Almeida et al 2012). A BBPI was shown to be involved in the tolerance to salt stress in wheat (Shan et al 2008) and Arabidopsis (Wang et al 2014).…”

Endonuclease com incompatibilidade heteroduplex para detectar mutação e variações genéticas de inibidores da tripsina em soja

“…A number of functions has been proposed for BBIs, including the regulation of protease activity during seed germination and the protection of plants from insects and microorganisms. Moreover, BBIs may also function as storage of sulfur amino acids (Barros et al, 2012;Cruz et al, 2013). Recent investigations have focused on its medicinal utility for suppressing both initiation and promotion stages of carcinogenesis (Rakashanda & Amin, 2013).…”

“…Recent investigations have focused on its medicinal utility for suppressing both initiation and promotion stages of carcinogenesis (Rakashanda & Amin, 2013). BBI protease inhibitors are double-headed serine protease inhibitors that bind both enzymes at two independent reactive sites with a network of highly conserved disulfide bridges (Barros et al, 2012).…”

“…Post-translational proteolysis indicates that BBI-EI is originated from BBI-DII. Thus, BBIs are grouped in three types with distinct characteristics: BBI-A, BBI-C, and BBI-D (Deshimaru et al, 2004;Wang et al, 2008;Barros et al, 2012).…”

Bowman-Birk Inhibitors from Legumes: Utilisation in Disease Prevention and Therapy

“…The BBI that are expressed in seeds are the products of multi-gene families. Several isoinhibitors have been identified in seeds of individual species [11,12]. The expression of distinct genes, together with the post-translational modifications of primary gene products, combine to give rise to the array of BBI-like variants described for many legume species.…”