In-silico characterization of phytochemicals identified from Vitex negundo (L) extract as potential therapy for Wnt-signaling proteins

Abstract

Background Colorectal cancer is the third most diagnosed disease in the world population and current chemotherapy has been used for targeting the cell proliferation and metastasizing ability of tumor cells. Potent chemotherapeutic drugs for colorectal cancer are capecitabine, fluorouracil, irinotecan, etc. which have toxic effects in normal tissues and adverse effects in multiple organs leading to major obstacles in clinical use. The aim of the study is the use of plant-derived compounds that improve the effectiveness of chemotherapeutics with lower and alleviate toxic side effects and reduce the risk of tumor progression. Results The current study is performed using Vitex negundo leaf which has been demonstrated to have positive effects against colorectal cancer. The use of computational approaches will help improve the identification and screening of lead molecules using AutoDock 4.2 and AutoDock Vina. Using computational approaches will help to improve lead identification and screening. Herein, we have retrieved six phytochemicals from published literature and investigated their inhibitory effect with Wnt-associated signaling proteins. Authentication of phytocompounds and Wnt-associated signaling proteins was done using AutoDock.4.2. Conclusions The results are screened based on the number of hydrogen bonds, binding energy, and interacting amino acids. The Isoorientin, luteolin, and Chrysophanol get the highest binding energy with target receptors. The binding energy is calculated with all target receptors from the range of − 6.0 to − 8.9 kcal/mol. The In-silico drug likeliness properties are predicted to be the best interacting compounds based on Lipinski Rule of 5 and ADMET analysis. Hence, we propose that Isoorientin, luteolin, and Chrysophanol are the potential inhibitors of Wnt signaling inhibitors, and preclinical studies are needed to confirm the promising therapeutic ability of colorectal cancer.

Design, Synthesis, and Anti-Inflammatory Activity of Some Coumarin Schiff Base Derivatives: In silico and in vitro Study

“…During the docking process, AutoDock combines the Lamarckian genetic algorithm and empirical free energy force fields to calculate the best-bound conformations and energy of the protein-ligand interactions. 69–73 Both glg and dlg files were generated using canonical Ubuntu 18.04.6 LTS (Bionic Beaver; https://releases.ubuntu.com/bionic ) as an open-source operating system by applying the Linux kernel and based on Debian. 74…”

Silymarin-Loaded Tin(IV) Nanoparticles Exhibit Enhanced Bioavailability and Antiproliferative Effects on Colorectal Cancer Cells

“…Following the heightened need for establishment of new effective curative options, recent attention has focused on the chemoprevention impact of phytochemicals such as vegetables, fruits, and medicinal plants . Based on the previously reported scientific evidence, there is a reverse association between consumption of natural products and the risk of colorectal cancer development. , …”

“…7 Based on the previously reported scientific evidence, there is a reverse association between consumption of natural products and the risk of colorectal cancer development. 8,9 Silymarin is derived from milk thistle (Silybum marianum) and comprises a mixture of flavonolignans (silybin, silydianin, and silychristin) and flavonoids (tamoxifen and quercetin). 10 It has been reported that silymarin exerts considerable antiinflammatory, anticarcinogenic, antioxidant, and proapoptotic properties.…”