2023
DOI: 10.3390/fishes9010005
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In Silico Evaluation of the PCR Performance of Different Tests for Detection of WSSV

Arturo Sánchez-Paz,
Trinidad Encinas-García,
Fernando Mendoza-Cano

Abstract: In this study, the primers of different protocols for the detection of White Spot Syndrome Virus (WSSV) were analyzed in silico to evaluate their potential performance in PCR. As with any biological entity, this virus evolves constantly. Thus, this analysis showed that a few primers, including those recommended by the World Organization for Animal Health (WOAH), might mismatch with some isolates of WSSV, specially with isolates more recently sequenced. Furthermore, a set of primers recommended by WOAH, showed … Show more

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