2018
DOI: 10.1159/000493496
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In Silico Identification and in Vitro Analysis of B and T-Cell Epitopes of the Black Turtle Bean (Phaseolus Vulgaris L.) Lectin

Abstract: Background/Aims: The incidence of lectin allergic disease is increasing in recent decades, and definitive treatment is still lacking. Identification of B and T-cell epitopes of allergen will be useful in understanding the allergen antibody responses as well as aiding in the development of new diagnostics and therapy regimens for lectin poisoning. In the current study, we mainly addressed these questions. Methods: Three-dimensional structure of the lectin from black turtle bean (Phaseolus vulgaris L.) was model… Show more

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Cited by 15 publications
(24 citation statements)
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“…Allergen-specific T cells play an important role in allergic reactions and are obvious targets for immunotherapy intervention in diseases [17]. Recently, Candreva et al [46] developed a new strategy for preventing milk allergy on the basis of oral administration of a soybean-derived peptide that was cross-reactive with bovine caseins.…”
Section: Discussionmentioning
confidence: 99%
See 2 more Smart Citations
“…Allergen-specific T cells play an important role in allergic reactions and are obvious targets for immunotherapy intervention in diseases [17]. Recently, Candreva et al [46] developed a new strategy for preventing milk allergy on the basis of oral administration of a soybean-derived peptide that was cross-reactive with bovine caseins.…”
Section: Discussionmentioning
confidence: 99%
“…The amino acid composition of T cell epitopes and the frequency were analyzed according to the methods of He et al [17] and Zheng et al [25].…”
Section: Epitope Amino Acid Composition Analysismentioning
confidence: 99%
See 1 more Smart Citation
“…Each protomer contains a CBS that specifically recognizes complex N-glycan chains, but does not recognize simple sugars, probably due to a particular conformation of the CBS. Both types of lectins from the white kidney bean [29,[54][55][56][57][58] and the black turtle bean [59,60] have been reported as allergens, and a detailed bioinformatics study of the B-and T-cell epitopes of PHA-E from black turtle bean has been performed [59]. Up to 7 IgE-binding B-epitopes (Figure 2B) and 3 T-epitopes (Figure 3) occur at the surface of the PHA-E tetramer.…”
Section: Homotetrameric Single-chain Lectinsmentioning
confidence: 99%
“…To date, however, the immunogenicity of lectins as allergenic proteins remains poorly known and difficult to foresee, because of the lack of information on the T-cell epitopes in these proteins. Except for PHA-E from black turtle bean [59], no information on the number and distribution of T epitopes is available for other potentially allergenic lectins. However, some information has been collected on the location and distribution of IgE-binding B-cell epitopic regions on the surface of a few lectins, especially legume lectins.…”
Section: Nictaba-related Lectinsmentioning
confidence: 99%