In silico prediction of conserved vaccine targets in Streptococcus agalactiae strains isolated from fish, cattle, and human samples

Pereira, Ulisses de Pádua; Soares, Siomar C.; Blom, Jochen; Leal, Carlos Augusto Gomes; Ramos, Rommel Thiago Jucá; Guimarães, Luís Carlos; Oliveira, Liliana; Almeida, Síntia; Hassan, Syed Shah; Santos, Anderson; Miyoshi, Anderson; Silva, A.; Tauch, Andreas; Barh, Debmalya; Azevedo, Vasco; Figueiredo, Henrique César Pereira

doi:10.4238/2013.august.12.6

Cited by 26 publications

(22 citation statements)

References 42 publications

(50 reference statements)

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“…Bioinformatic analyses of Sip reveal that there is a signal peptide at the N-terminus, followed by a LysM petidoglycan interaction domain. The phylogenetic relationship of Sip in GBS isolates shows that Sip has a high homology in humans, bovines and tilapia [15]. The whole Sip of GBS isolated from humans and bovines was expressed successfully in BL21(DE3) transformed with pET32a-sip [12,27]; but when the BL21 (DE3) host was used in conjunction with pET vectors, some toxic proteins could not be successfully reproduced [28].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A recombinant truncated surface immunogenic protein (tSip) plus adjuvant FIA confers active protection against Group B streptococcus infection in tilapia

Wang

Xiao³

et al. 2014

Vaccine

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Section: Discussionmentioning

confidence: 99%

“…Sip is an attractive protein for a GBS serotype independent subunit vaccine because it is the only protective protein in the core genome and has been isolated in fish, cattle, and human [13][14][15]. This protein also protects mice against GBS isolated from humans and cattle [12,16,17].…”

Section: Introductionmentioning

confidence: 97%

A recombinant truncated surface immunogenic protein (tSip) plus adjuvant FIA confers active protection against Group B streptococcus infection in tilapia

Wang

Xiao³

et al. 2014

Vaccine

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“…The Vaxign execution pipeline is able to predict antigen cellular localization, adhesion, epitope binding to MHC class I and class II, and sequence similarities to human, mouse and/or pig proteins. Vaxign has successfully been tested in prediction of vaccine candidates for many different pathogens such as Brucella [5-7] , uropathogenic E. coli [4], Francisella [8], Streptococcus agalactiae [9] and human herpes simplex virus [10]. The experimental verification of Vaxign-predicted results [7] confirms the accuracy of the Vaxign vaccine candidate prediction.…”

Section: Progresses and Challengesmentioning

confidence: 94%

Ontology-supported research on vaccine efficacy, safety and integrative biological networks

2014

Expert Review of Vaccines

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Summary While vaccine efficacy and safety research has dramatically progressed with the methods of in silico prediction and data mining, many challenges still exist. A formal ontology is a human- and computer-interpretable set of terms and relations that represent entities in a specific domain and how these terms relate to each other. Several community-based ontologies (including the Vaccine Ontology, Ontology of Adverse Events, and Ontology of Vaccine Adverse Events) have been developed to support vaccine and adverse event representation, classification, data integration, literature mining of host-vaccine interaction networks, and analysis of vaccine adverse events. The author further proposes minimal vaccine information standards and their ontology representations, ontology-based linked open vaccine data and meta-analysis, an integrative One Network (“OneNet”) Theory of Life, and ontology-based approaches to study and apply the OneNet theory. In the Big Data era, these proposed strategies provide a novel framework for advanced data integration and analysis of fundamental biological networks including vaccine immune mechanisms.

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“…The underlying reasons for this general tendency of the terrestrial isolates to cause higher mortality in P1 and P2 compared to the piscine isolates are still unclear. However, recent studies have shown that some of the major virulence factors found in human and bovine isolates are absent from the ST260-261 GBS piscine lineages (Delannoy et al, 2013;Pereira et al, 2013b;Rosinski-Chupin et al, 2013). This needs to be confirmed in our collection of Australian ST-261 piscine isolates that have been used in this study.…”

Section: Phagocytosis and Downstream Cellular Antimicrobial Response mentioning

confidence: 99%

“…Comparison of the bacterial pan-genome of different strains isolated from different animals can revealed conserved antigen targets than can be used to better inform during bacterial vaccine design (Maione et al, 2005;Pereira et al, 2013b;Schubert-Unkmeir and Christodoulides, 2013;Tettelin, 2009). …”

Section: Advent Of the Latest Next Generation Sequencing (Ngs) Technomentioning

confidence: 99%

On the origin of Group B Streptococcus from disease outbreaks in wild marine fish in Australia

Deboutteville¹

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Since 2007, 96 wild Queensland groupers, Epinephelus lanceolatus, were found dead from Karumba (Gulf of Carpentaria) in Northern Territory to Brisbane in southern Queensland, Australia. In twelve cases, Streptoccocus agalactiae (Group B Streptococcus, GBS) was isolated in pure culture from eyes and internal organs. GBS has been also isolated from other marine species (Javelin grunter, Giant catfish and Squaretail mullet) and caused an outbreak in three species of wild stingrays (Eastern shovelnose ray, Mangrove whipray and Estuary ray) in a public aquarium also in Queensland. These findings have raised public and industry concern over threats to human health from potential transmission between fish and humans, over environmental impacts on other aquatic animals, and over the potential threat posed to the growing Australian aquaculture industry in which, to date, there have been no reported cases of this disease in spite of the devastation caused to the industry overseas. Therefore, my thesis set out to determine whether GBS isolated from dead wild fish in Australia is indeed virulent in Queensland grouper and causative of mortality, to try to identify from where these infections in wild fish originated, to determine potential risk to human health and to other aquatic animals, and to begin to identify mechanisms by which these Australian isolates may cause fatal disease in Queensland grouper. This information may be employed in future risk assessment of potential human transfer, in mitigating further outbreaks in wild fish through understanding origin of infection, and in future development of preventative measures such as vaccination for farmed fish.In order to confirm that S. agalactiae was the cause of the mortality in wild fish, experimental challenges were conducted by injection, immersion and through the oral route. To better understand why these strains of GBS are pathogenic in fish, we conducted a series of cellular immune assays using juvenile Queensland grouper head-kidney leucocytes in the presence of different piscine and terrestrial isolates. Whole-genome sequencing analysis of the piscine and terrestrial isolates provided information on potential origin and potential for transfer between species, and also informed us on critical virulence factors that could be used during pathogenesis of GBS. Moreover, genome analysis gave intriguing insight into the evolution and adaption of GBS to fish as a host and to life in the aquatic environment. To determine putative origin of GBS in wild fish in Australia, multilocus sequence typing (MLST) and capsular polysaccharide (CPS) were derived from whole-genome sequences and revealed that all piscine isolates from Australia were serotype Ib and fell into ST-261 lineage first identified in 1986 from Tilapia in Israel. Overseas, ST-261 has only been found in fish and poikilotherm animals, and has never been associated with humans. High similarity and identical point mutations in the capsular operon of the piscine isolates suggests a common source of infection...

show abstract

In silico prediction of conserved vaccine targets in Streptococcus agalactiae strains isolated from fish, cattle, and human samples

Cited by 26 publications

References 42 publications

A recombinant truncated surface immunogenic protein (tSip) plus adjuvant FIA confers active protection against Group B streptococcus infection in tilapia

A recombinant truncated surface immunogenic protein (tSip) plus adjuvant FIA confers active protection against Group B streptococcus infection in tilapia

Ontology-supported research on vaccine efficacy, safety and integrative biological networks

On the origin of Group B Streptococcus from disease outbreaks in wild marine fish in Australia

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