In silico prediction of microRNAs on fluoride induced sperm toxicity in mice

Raghunath, Azhwar; Jeyabaskar, Dhivyalakshmi; Sundarraj, Kiruthika; Panneerselvam, Lakshmikanthan; Perumal, Ekambaram

doi:10.1016/j.fct.2016.03.005

Cited by 22 publications

(8 citation statements)

References 63 publications

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“…The association between miR-122-5p and ALDOA has been reported and the influence of this modulation was predicted to involve in the progression of some diseases. In silico prediction of microRNAs conducted by Raghunath et al, the Aldoa mRNA:miR-122-5p interaction in the liver was confirmed using various methods like reporter assay, western blot and qPCR [24]. MiRNAs analysis ashowed the significant down-regulation of three miRNAs (miR-122, miR-451 and miR-27) in the pathogenesis of diet-induced nonalcoholic fatty liver disease and ALDOA was regarded as one of the miR-122 targets [25].…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 99%

miR-122-5p Inhibits the Proliferation, Invasion and Growth of Bile Duct Carcinoma Cells by Targeting ALDOA

Liu

Zhang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Bile duct cancer, although not among the most common tumors, still accounts for more and more worldwide deaths each year. By attempting to verify an overexpression of ALDOA in cholangiocarcinoma tissues and cells and explore the underlying molecular mechanism regulated by miR-122-5p, this study was designed to provide a potential molecular target in bile duct cancer treatment. Methods: Western blot and immunohistochemistry were performed to detect the ALDOA protein level in duct carcinoma tissues. The transfection efficiency was confirmed by western blot and/or RT-qPCR assay. The proliferation of bile duct carcinoma cells was determined by MTT and colony formation assay. The invasion ability of bile duct carcinoma cells was evaluated with Transwell invasion assay. Flow cytometry detected cell apoptosis of bile duct carcinoma cells. The miRNAs which modulate ALDOA were filtrated from bioinformatics software and clinical specimens. The target relationship was confirmed by dual luciferase reporter assay. Furthermore, a xenograft model was completed to verify the impact of miRNA on inhibition growth of bile duct carcinoma cells. Results: ALDOA was found up-regulated in bile duct carcinoma tissues and cells. Knockdown of ALDOA promoted the apoptosis of cells and inhibited the proliferation and invasion of bile duct carcinoma cells. Bioinformatics and clinical specimens indicated the negative correlation and targeted regulation between miR-122-5p and ALDOA. By down-regulating ALDOA, overexpression of miR-122-5p appeared to promote cell apoptosis and significantly inhibit cell proliferation, invasion in vitro and suppress the tumor growth in vivo. Conclusion: miR-122-5p inhibited proliferation and invasion of bile duct carcinoma cells and promoted cell apoptosis by targeting ALDOA expression.

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Section: Cellular Physiology and Biochemistrymentioning

confidence: 99%

miR-122-5p Inhibits the Proliferation, Invasion and Growth of Bile Duct Carcinoma Cells by Targeting ALDOA

Liu

Zhang

et al. 2018

Cell Physiol Biochem

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“…Fluorosis is a slow and progressive disease that affects human health. It is well known that fluoride can disrupt oxidative stress, endoplasmic reticulum stress [ 56 ], glycolysis [ 57 ], and nerve conduction [ 58 ]. Severe skeletal fluorosis is accompanied by chronic pain and even disability for a long time.…”

Section: Discussionmentioning

confidence: 99%

In Silico Analysis of Ferroptosis-Related Genes and Its Implication in Drug Prediction against Fluorosis

Liu

et al. 2023

IJMS

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Fluorosis is a serious global public health problem. Interestingly, so far, there is no specific drug treatment for the treatment of fluorosis. In this paper, the potential mechanisms of 35 ferroptosis-related genes in U87 glial cells exposed to fluoride were explored by bioinformatics methods. Significantly, these genes are involved in oxidative stress, ferroptosis, and decanoate CoA ligase activity. Ten pivotal genes were found by the Maximal Clique Centrality (MCC) algorithm. Furthermore, according to the Connectivity Map (CMap) and the Comparative Toxicogenomics Database (CTD), 10 possible drugs for fluorosis were predicted and screened, and a drug target ferroptosis-related gene network was constructed. Molecular docking was used to study the interaction between small molecule compounds and target proteins. Molecular dynamics (MD) simulation results show that the structure of the Celestrol–HMOX1 composite is stable and the docking effect is the best. In general, Celastrol and LDN-193189 may target ferroptosis-related genes to alleviate the symptoms of fluorosis, which may be effective candidate drugs for the treatment of fluorosis.

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“…We employed a network pharmacology approach to determine potential miRNAs associated with the hub genes (Jiang, Lu, et al, 2020) through database screening, commenced with mRNA complementarity to miR-NA's seed region that subsequently moves on to the 23 bases that exhibit legitimate interactions (Raghunath et al, 2016). Experimentally validated miRNA-mRNA interactions were predicted from an online repository, mirTarbase, which encompasses reporter assay, Western blot, qPCR, microarrays, NGS, and other approaches.…”

Section: Discussionmentioning

confidence: 99%

Identification of therapeutic miRNAs from the arsenic induced gene expression profile of hepatocellular carcinoma

Mukherjee

Acharya²,

Singh

et al. 2022

Chem Biol Drug Des

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Hepatocellular carcinoma (HCC) is the most common primary liver cancer, with a rising worldwide burden due to a lack of efficient treatment techniques and diagnosis after it has metastasized. Therefore, small non‐coding RNA (miRNAs) as protein translation inhibitors are gaining attention that degrades or suppress specific gene transcripts, making it a prime strategy for oncogenes or tumor suppression. Systematic research with miRNAs in combination with Arsenic, which has been employed as a drug to treat several diseases, including cancer, was focused on cellular responses through interacting with multiple biological targets. The differential gene expression of the DNA microarray dataset (GSE48441) revealed the association of sterol, cholesterol, and lipid metabolic processes. With the aid of the network pharmacology approach, hsa‐mir‐335‐5p was uncovered to negatively regulate the important nodes driving the transport and utilization of essential compounds for the rapid growth and proliferation of cancer cells. The binding energies of the duplexes were validated by the minimal free energies of the mRNAs for hsa‐mir‐335‐5p, indicating energetically desirable binding association. The molecular interactions between hsa‐mir‐335‐5p, which interacts with the Argonaute protein in the RNA induced silencing complex, and the target‐specific genes were also investigated, revealing its susceptibility to be employed in in vitro studies.

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In silico prediction of microRNAs on fluoride induced sperm toxicity in mice

Cited by 22 publications

References 63 publications

miR-122-5p Inhibits the Proliferation, Invasion and Growth of Bile Duct Carcinoma Cells by Targeting ALDOA

miR-122-5p Inhibits the Proliferation, Invasion and Growth of Bile Duct Carcinoma Cells by Targeting ALDOA

In Silico Analysis of Ferroptosis-Related Genes and Its Implication in Drug Prediction against Fluorosis

Identification of therapeutic miRNAs from the arsenic induced gene expression profile of hepatocellular carcinoma

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