In silico promoter characterization of PR genes and expression analysis in transgenic tomato plants expressing OsRGLP1

Ohwofasa, Aghogho; Perveen, Sumera; Khan, Tehmina Ahsen; Ijaz, Bushra; Yasmin, Tayyaba

doi:10.30848/pjb2021-5(36)

Cited by 2 publications

(2 citation statements)

References 31 publications

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“…to get insight into their function and regulatory mechanism. Recently, in silico promoter analysis of 3 pathogenesis-related (PR1, 2, 3) and OsRGLP1 genes promoters revealed several common cis-elements (Ohwofasa et al, 2021). The highest number of cis-elements in VvGLP6 represents its importance in various processes.…”

Section: Promoter Analysismentioning

confidence: 99%

Analysis of Germin-like protein genes family in Vitis vinifera (VvGLPs) using various in silico approaches

Ilyas

Rahman²,

Khan³

et al. 2024

Braz. J. Biol.

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Germin-like proteins (GLPs) play an important role against various stresses. Vitis vinifera L. genome contains 7 GLPs; many of them are functionally unexplored. However, the computational analysis may provide important new insight into their function. Currently, physicochemical properties, subcellular localization, domain architectures, 3D structures, N-glycosylation & phosphorylation sites, and phylogeney of the VvGLPs were investigated using the latest computational tools. Their functions were predicted using the Search tool for the retrieval of interacting genes/proteins (STRING) and Blast2Go servers. Most of the VvGLPs were extracellular (43%) in nature but also showed periplasmic (29%), plasma membrane (14%), and mitochondrial- or chloroplast-specific (14%) expression. The functional analysis predicted unique enzymatic activities for these proteins including terpene synthase, isoprenoid synthase, lipoxygenase, phosphate permease, receptor kinase, and hydrolases generally mediated by Mn+ cation. VvGLPs showed similarity in the overall structure, shape, and position of the cupin domain. Functionally, VvGLPs control and regulate the production of secondary metabolites to cope with various stresses. Phylogenetically VvGLP1, -3, -4, -5, and VvGLP7 showed greater similarity due to duplication while VvGLP2 and VvGLP6 revealed a distant relationship. Promoter analysis revealed the presence of diverse cis-regulatory elements among which CAAT box, MYB, MYC, unnamed-4 were common to all of them. The analysis will help to utilize VvGLPs and their promoters in future food programs by developing resistant cultivars against various biotic (Erysiphe necator and in Powdery Mildew etc.) and abiotic (Salt, drought, heat, dehydration, etc.) stresses.

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Section: Promoter Analysismentioning

confidence: 99%

Analysis of Germin-like protein genes family in Vitis vinifera (VvGLPs) using various in silico approaches

Ilyas

Rahman²,

Khan³

et al. 2024

Braz. J. Biol.

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“…Despite the several breakthroughs in tomato fruit improvement through traditional and molecular breeding, still, many known cultivars fnd humid environments unfavorable. In such environments, yield is reduced due to high fower abortion rates, disease incidence, and rapid postharvest fruit decay [8,9].…”

Section: Introductionmentioning

confidence: 99%

Hybrid Vigor and Heritability Estimates in Tomato Crosses Involving Solanum lycopersicum × S. pimpinellifolium under Cool Tropical Monsoon Climate

Ene

Abtew

Oselebe

et al. 2023

International Journal of Agronomy

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High humidity is a major constraint to increased tomato fruit production in a cool tropical monsoon climate. However, the genetic variation observed in Solanum pimpinellifolium makes it a good gene donor for breeding tomato cultivars capable of thriving under high humidity. The objective of this study was to estimate heterosis, heritability for higher yield, and to assess the adaptability of the genotypes to humid conditions. Genotypes were raised from five morphologically divergent parents, viz., wild parent (W)–“LA2093,” “CLN2498D” (D), “CLN2417H” (H), “Tima” (T), and “UC Dan INDIA” (U). The F1s were generated by biparental mating design using “LA2093” as a common pollen donor that was selfed to produce F2s and backcrossed to both parents to obtain BC1s and BC2s. The trial was laid out in a randomized complete block design with three replicates. Data were collected on selected yield-influencing traits and analyzed. “D × W” and “U × W” hybrids showed significant positive better parent heterosis for fruit weight per plant (30.4% and 35.5%) and total fruit yield (48.6% and 26.9%), respectively. The additive variance was higher than dominance variance for all the traits, including total fruit yield in all hybrids viz., “H × W,” “D × W,” “T × W,” and “U × W.” High narrow sense heritability estimate of ≥60% was observed in “D × W” and “U × W” hybrids for the majority of the floral and fruit traits including total fruit yield. This makes the improvement of “D × W” and “U × W” hybrids by direct selection advantageous. Hence, the adoption of selection for the affected traits in subsequent tomato breeding programs would enhance fruit yield and adaptability to humid environments.

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In silico promoter characterization of PR genes and expression analysis in transgenic tomato plants expressing OsRGLP1

Cited by 2 publications

References 31 publications

Analysis of Germin-like protein genes family in Vitis vinifera (VvGLPs) using various in silico approaches

Analysis of Germin-like protein genes family in Vitis vinifera (VvGLPs) using various in silico approaches

Hybrid Vigor and Heritability Estimates in Tomato Crosses Involving Solanum lycopersicum × S. pimpinellifolium under Cool Tropical Monsoon Climate

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