In silico search, chemical characterization and immunogenic evaluation of amino-terminated G4-PAMAM-HIV peptide complexes using three-dimensional models of the HIV-1 gp120 protein

Rodríguez-Fonseca, Rolando Alberto; Bello, Martiniano; Muñoz‐Fernández, María Ángeles; Jiménez, José Luís; Rojas-Hernández, Saúl; Fragoso-Vázquez, Manuel Jonathan; Gutiérrez-Sánchez, Mara; Rodrigues, João; Cayetano-Castro, N.; Borja-Urby, Raúl; Rodríguez‐Cortés, Octavio; García-Machorro, Jazmín; Correa‐Basurto, José

doi:10.1016/j.colsurfb.2019.01.034

Cited by 23 publications

(19 citation statements)

References 51 publications

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“…tissue adhesives) are but a few industries that could benefit from electrocuring designs [5][6][7][8][9] . In addition, applications of Voltaglue could also be explored in the fields where PAMAM has been exploited such as-stimuli-responsive biomaterials, electrochemical sensors, conductive polymers, and controlled chemical releaseall applications that can benefit from voltage-activated adhesion [10][11][12][13][14][15][16][17] .…”

Section: Discussionmentioning

confidence: 99%

“…Electrochemical activation of Voltaglue is possible with more robust biocompatible substrates, and this will be part of our future work. Microelectronic fabrication (e.g., flip-chips), construction (e.g., window sealants), and medicine (e.g., tissue adhesives) are but a few industries that could benefit from electrocuring designs. − In addition, applications of Voltaglue could also be explored in fields where PAMAM has been exploited, such as stimuli-responsive biomaterials, electrochemical sensors, conductive polymers, and controlled chemical release, all applications that can benefit from voltage-activated adhesion. − …”

Section: Discussionmentioning

confidence: 99%

“…Voltaglue is an example of voltage-activated adhesives, in which aryl-diazirine (electrochemical cross-linker) is grafted onto the surface of a polyamidoamine (PAMAM) dendrimer. PAMAM dendrimers are commercially available in a number of sizes and a choice of surface functionalizations. − Aryl-diazirine is an ambient stable precursor that can be activated by heat (>100 °C), UVA, or voltage.…”

Section: Introductionmentioning

confidence: 99%

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Synergistic Voltaglue Adhesive Mechanisms with Alternating Electric Fields

et al. 2020

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Voltage-activated adhesion is a relatively new discovery that relies on direct currents for initiation of crosslinking. Previous investigations have found that direct currents are linearly correlated to the migration rates of electrocuring, but this is limited by high voltages exceeding 100 V with instances of incomplete curing of voltage-activated adhesives on semiconducting substrates. Practical applications of electrocuring would benefit from lower voltages to mitigate high voltage risks, especially with regard to potential medical applications. Alternative electrocuring strategies based on alternating current (AC), electrolyte ionic radius, and temperature are evaluated herein. Square waveform AC electric fields are hypothesized to initiate a two-sided curing progression of voltage-activated adhesive (PAMAM-g-diazirine aka Voltaglue), where initiation occurs at the cathode terminal. Structure-activity relationships of AC frequency at currents of 1-3 mA are evaluated against direct currents, migration rate, storage modulus, and lap shear adhesion on ex-vivo tissue mimics. Numerous improvements in electrocuring are observed with AC stimulation versus DC, including a 35 % decrease in maximum voltage, 180 % improvement in kinetic rates, and 100 % increase in lap shear adhesion at 2 mA. Li + ion electrolytes and curing at 4 o C shift curing kinetics by +104 % and -22 % with respect to the control ion (Na + ion at 24 o C), suggesting electrolyte migration is the rate limiting step. Li + ion electrolytes and curing at 50 o C improves storage modulus by 110% and 470 % respectively. Further evaluations of electrocured matrices with 19 F NMR, solid-state NMR and infrared spectroscopy provide insights into the probable crosslinking mechanisms.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synergistic Voltaglue Adhesive Mechanisms with Alternating Electric Fields

et al. 2020

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“…Due to such features, physicochemical and biological properties can be customized through the introduction of different functionalized surface moieties 170 . For instance, intranasal immunization of the HIV-1 gp120 peptide complexed with fourth-generation polyamidoamine dendrimer (G4-PAMAM) induced peptide-specific IgG and IgA responses in the serum, nasal wash, and vaginal fluid of mice 171 .…”

Section: Nanotechnology-assisted Covid-19 Mucosal Vaccinementioning

confidence: 99%

Mucosal delivery of nanovaccine strategy against COVID-19 and its variants

Lee

Khang

2023

Acta Pharmaceutica Sinica B

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“…16 One of the most promising approaches is the physically entrapment of a drug inside a dendrimer-based nanocarrier using non-covalent interactions. 17, 18 Dendrimers are strong candidates because they offer structural stability, high carrying capacity of molecules, water solubility, modifiable surface functionality, available internal cavities, pH dependence properties and a high capacity to cross cell barriers together with an ease of synthesis or commercial availability. 19 Most of the previous studies have focused on PAMAM, among all dendrimers and hydroxyl-terminated PAMAM dendrimer has been additionally proven to be a non-cytotoxic variant compared with the original amino-terminated PAMAM dendrimer that shows a slight cytotoxic effect.…”

Section: Introductionmentioning

confidence: 99%

Unveiling the G4-PAMAM capacity to bind and protect Ang-(1-7) bioactive peptide

América

Asgharpour

Correa‐Basurto

et al. 2022

Preprint

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New therapies that allow natural healing processes are required. Such as the endogenous peptide called Angiotensin-(1-7), a safe and effective drug, which is able to re-balance the Renin-Angiotensin system affected during several pathologies, including the new COVID-19; cardiovascular, renal, and pulmonary disease; diabetes; neuropathic pain; Alzheimer and cancer. However, one of the limiting factors for its application is its unfavorable pharmacokinetic profile. In this work, we propose the coupling of Angiotensin-(1-7) to PAMAM dendrimers in order to evaluate the capacity of the nanocarrier to improve isolated peptide features and to gain insight into the structural as well as the energetic basis of its molecular binding. The In Silico tests were performed in acidic and neutral pH conditions as well as amino-terminated and hydroxyl-terminated PAMAM dendrimers. High-rigor computational approaches, such as molecular dynamics and metadynamics simulations were used. We found that, at neutral pH, PAMAM dendrimers with both terminal types are able to interact stably with 3 Angiotensin-(1-7) peptides through ASP1, TYR4 and PRO7 critical amino acids, however, there are some differences in the binding sites of the peptides. In general, they bind on the surface in the case of the hydroxyl-terminated compact dendrimer and in the internal zone in the case of the amino-terminated open dendrimer. At acidic pH, PAMAM dendrimers with both terminal groups are still able to interact with peptides either internalized or in its periphery, however, the number of contacts, the percentage of coverage and the number of HBs are lesser than at neutral pH, suggesting a state for peptide release. In summary, amino-terminated PAMAM dendrimer showed slightly better features to bind, load and protect Angiotensin-(1-7) peptides.

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In silico search, chemical characterization and immunogenic evaluation of amino-terminated G4-PAMAM-HIV peptide complexes using three-dimensional models of the HIV-1 gp120 protein

Cited by 23 publications

References 51 publications

Synergistic Voltaglue Adhesive Mechanisms with Alternating Electric Fields

Synergistic Voltaglue Adhesive Mechanisms with Alternating Electric Fields

Mucosal delivery of nanovaccine strategy against COVID-19 and its variants

Unveiling the G4-PAMAM capacity to bind and protect Ang-(1-7) bioactive peptide

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