In silico structural-functional characterization of three differentially expressed resistance gene analogs identified in Dalbergia sissoo against dieback disease reveals their role in immune response regulation

Abstract

Plant immunity includes enemy recognition, signal transduction, and defensive response against pathogens. We experimented to identify the genes that contribute resistance against dieback disease to Dalbergia sissoo, an economically important timber tree. In this study, we investigated the role of three differentially expressed genes identified in the dieback-induced transcriptome in Dalbergia sissoo. The transcriptome was probed using DOP-rtPCR analysis. The identified RGAs were characterized in silico as the contributors of disease resistance that switch on under dieback stress. Their predicted fingerprints revealed involvement in stress response. Ds-DbRCaG-02-Rga.a, Ds-DbRCaG-04-Rga.b, and Ds-DbRCaG-06-Rga.c showed structural homology with the Transthyretin-52 domain, EAL associated YkuI_C domain, and Src homology-3 domain respectively, which are the attributes of signaling proteins possessing a role in regulating immune responses in plants. Based on in-silico structural and functional characterization, they were predicted to have a role in immune response regulation in D. sissoo.

Genome-Wide in Silico Analysis of the Heat Shock Protein (HSP) Family in Vigna Species: Insights into Molecular Mechanisms of Stress Response

“…Gene that responds to these stresses play a vital role in plant physiology (Ahanger et al, 2017). In silico Genome-wide analysis of these genes will provide an opportunity to develop resistant-linked markers and genetic maps and phylogenetic relationship for significantly studying the plant genome (Chen et al, 2021;Ijaz et al, 2023b). However, these genes are controlled by several transcription factors that regulate their functioning in stressfull environment.…”