2021
DOI: 10.1186/s12859-021-04378-z
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In silico vaccine design and epitope mapping of New Delhi metallo-beta-lactamase (NDM): an immunoinformatics approach

Abstract: Background Antibiotic resistance is a global health crisis. The adage that “prevention is better than cure” is especially true regarding antibiotic resistance because the resistance appears and spreads much faster than the production of new antibiotics. Vaccination is an important strategy to fight infectious agents; however, this strategy has not attracted sufficient attention in antibiotic resistance prevention. New Delhi metallo-beta-lactamase (NDM) confers resistance to many beta-lactamases… Show more

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Cited by 15 publications
(7 citation statements)
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“…The CAI and GC content of the long nucleotide sequence of 2772 bp was evaluated to optimize the vaccine construct. Better expression (transcription and translation) in organisms requires a GC content between 30 and 70% to be optimal, while a CAI value should be higher than 0.8 to 1 [93,94]. The GC content and CAI values of the optimized nucleotide sequence obtained from the Jcat server were 50.180% and 0.9913, respectively.…”
Section: Codon Adaptation and In Silico Cloningmentioning
confidence: 99%
“…The CAI and GC content of the long nucleotide sequence of 2772 bp was evaluated to optimize the vaccine construct. Better expression (transcription and translation) in organisms requires a GC content between 30 and 70% to be optimal, while a CAI value should be higher than 0.8 to 1 [93,94]. The GC content and CAI values of the optimized nucleotide sequence obtained from the Jcat server were 50.180% and 0.9913, respectively.…”
Section: Codon Adaptation and In Silico Cloningmentioning
confidence: 99%
“…Chakraborty and colleagues [ 99 ], adopted immunoinformatic approach to develop peptide-based vaccines against SARS-CoV-2. Fathollahi and colleagues also engaged in immunoinformatics approaches for vaccine design [ 100 ].…”
Section: Resultsmentioning
confidence: 99%
“…C-ImmSim server is an online, dynamic immune simulation server that uses the Celada-Seiden model to estimate the adaptive immune humoral and cellular response against a given antigen. 43 In our study, four injections through 28 days with 63 stimulation steps (1-time step equal to 8 hours) were considered to analyze immune responses. The production of antibodies, cytokines, and interferon after injection of the vaccine protein was calculated.…”
Section: Methodsmentioning
confidence: 99%