In situ assessment of the saliva effect on enamel morphology after microabrasion technique

Abstract

Aim: This study evaluated saliva effects on enamel morphology surface after microabrasion technique. Methods: Enamel blocks (16 mm 2) obtained from bovine incisors were divided into 9 groups as follows: one control group (no treatment), four groups with microabrasion treatment using 35% phosphoric acid and pumice (H 3 PO 4 +Pum) and other four groups treated with 6.6% hydrochloric acid and silica (HCl+Sil). One group of each treatment was submitted to 4 frames of saliva exposure: without exposure, 1-h exposure, 24-h exposure, and 7-days exposure on in situ regimen. Nineteen volunteers (n=19), considered as statistical blocks, used an intraoral appliance containing the specimens, for 7 days. Enamel roughness (Ra) was tested before and after treatment, and after saliva exposure. Confocal laser scanning microscopy (CLSM) was used to evaluate qualitatively the enamel morphology. Results: All groups exhibited increased Ra after microabrasion. With regards to saliva exposure, the treatment with HCl+Sil presented more susceptibility to the saliva action, but no period of time was efficient in re-establishing this characteristic compared with the control group. CLSM analysis showed reduction of the micro-abrasive damages during the experimental times. Conclusions: Seven days of saliva exposure were not sufficient for the treated enamel to reach its normal characteristics compared with the control group.

Tooth enamel properties and morphology after microabrasion: an in situ study

“…For the H 3 PO 4 + pumice treatment, a decrease of mineral loss was observed only after 24 h of saliva exposure; the mineral composition never matched that of the control group, not even after the 7‐day in situ regimen. These results are in agreement Pini et al . in relation to the roughness analysis.…”

“…Moreover, the mechanical application recommended for the microabrasion technique can emphasize these effects . Enamel microhardness and roughness can be influenced by a combination of the abovementioned factors, as described in previous studies . However, most of these studies have not considered the protective capacity of the oral environment, due to the presence of saliva and its components.…”

“…8,9 Enamel microhardness and roughness can be influenced by a combination of the abovementioned factors, as described in previous studies. [12][13][14][15][16][17][18] However, most of these studies have not considered the protective capacity of the oral environment, due to the presence of saliva and its components.…”

Resolution of a fluorosis case through the association of minimally invasive techniques

“…The microabrasive agents used for the microabrasion procedure are 6% hydrochloric acid associated with silicon carbide, which is found commercially, or a combination of pumice stone with 37% phosphoric acid, performed in-office 5,6 .…”

Enamel morphology after microabrasion with experimental compounds

“…Recently, scientific studies have been performed to improve microabrasion and to know its effects on enamel. [ 8 9 10 11 12 ] Initially, the acid used for microabrasion was 18% HCl; today, it is recognized that this concentration is considered erosive and toxic. Currently, the same acid is employed at a lower concentration and containing silica in commercial formulations.…”