In Situ Autofluorescence Spectroelectrochemistry for the Study of Microbial Extracellular Electron Transfer

Schmidt, Igor; Pieper, Alexander; Wichmann, Hilke; Bunk, Boyke; Huber, Katharina J.; Overmann, Jörg; Walla, Peter Jomo; Schröder, Uwe

doi:10.1002/celc.201700675

Cited by 14 publications

(11 citation statements)

References 31 publications

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“…Among the electrochemically active bacteria, Geobacter sulfurreducens and Shewanella oneidensis are most commonly studied. Especially G. sulfurreducens or its close relative G. anodireducens is well-known in bioelectrochemical research, since this bacterium is found highly enriched on anodic biofilms derived from municipal wastewater in MFCs or MECs, indicating its superior ability to perform extracellular electron transfer over other species (Lovley, 2006; Harnisch et al, 2011; Schmidt et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Long-Term Behavior of Defined Mixed Cultures of Geobacter sulfurreducens and Shewanella oneidensis in Bioelectrochemical Systems

Engel

Schattenberg

Dohnt

et al. 2019

Front. Bioeng. Biotechnol.

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This work aims to investigate the long-term behavior of interactions of electrochemically active bacteria in bioelectrochemical systems. The electrochemical performance and biofilm characteristics of pure cultures of Geobacter sulfurreducens and Shewanella oneidensis are being compared to a defined mixed culture of both organisms. While S. oneidensis pure cultures did not form cohesive and stable biofilms on graphite anodes and only yielded 0.034 ± 0.011 mA/cm 2 as maximum current density by feeding of each 5 mM lactate and acetate, G. sulfurreducens pure cultures formed 69 μm thick, area-wide biofilms with 10 mM acetate as initial substrate concentration and yielded a current of 0.39 ± 0.09 mA/cm 2 . Compared to the latter, a defined mixed culture of both species was able to yield 38% higher maximum current densities of 0.54 ± 0.07 mA/cm 2 with each 5 mM lactate and acetate. This increase in current density was associated with a likewise increased thickness of the anodic biofilm to approximately 93 μm. It was further investigated whether a sessile incorporation of S. oneidensis into the mixed culture biofilm, which has been reported previously for short-term experiments, is long-term stable. The results demonstrate that S. oneidensis was not stably incorporated into the biofilm; rather, the planktonic presence of S. oneidensis has a positive effect on the biofilm growth of G. sulfurreducens and thus on current production.

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Section: Introductionmentioning

confidence: 99%

Long-Term Behavior of Defined Mixed Cultures of Geobacter sulfurreducens and Shewanella oneidensis in Bioelectrochemical Systems

Engel

Schattenberg

Dohnt

et al. 2019

Front. Bioeng. Biotechnol.

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“…Based on the specific composition of the cellular components, bacterial species can be identified by means of their distinct Raman spectra. 20,21 Since previous studies in our lab showed a dominance of Geobacter species under the chosen environmental conditions, 22 we compared the Raman spectra depicted in Fig. 2A with the Raman spectrum of a pure culture of Geobacter sulfurreducens (Fig.…”

Section: Biofilms Grown On Copper Contain Copper Sulphidesmentioning

confidence: 99%

Copper-bottomed: electrochemically active bacteria exploit conductive sulphide networks for enhanced electrogeneity

Beuth

Pfeiffer

Schröder

2020

Energy Environ. Sci.

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“…This finding is of great importance for understanding biomembrane‐related phenomena such as neurotransmission and respiration as well as the delivery of ionic drugs into the cell. Fluorescence spectroelectrochemical measurements were also used to evaluate the extracellular electron transfer (EET) of electrochemically active microbial biofilms and the potential‐dependent conformational changes in redox enzymes involved in these processes [125] . In addition, this technique provides information on the chemical nature and kinetic parameters of EET components.…”

Section: Biospectroelectrochemistry Through Membranes and Cellsmentioning

confidence: 99%

In Situ and Operando Techniques for Investigating Electron Transfer in Biological Systems

et al. 2020

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When bioelectrochemistry meets other analytical techniques for in situ experiments, new possibilities for understanding the nature of charge‐transfer reactions are provided. Despite progress in recent years in analytical instrumentation, a number of key issues remains for bioelectrochemistry, in terms of the mechanistic description of the surface reactions involved in biomolecules’ electron transfer. The main challenges of these techniques are the concomitant detection of electron transfer and molecular alteration as well as the development of suitable bioelectrodes. This review discusses the most recent and emerging in situ and operando electrochemical methods used to study biological systems such as redox proteins, nucleic acids, small biomolecules, cells, and biomembranes, focusing on electrochemical methods coupled with spectroscopic, spectrometric, and microscopic techniques.

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In Situ Autofluorescence Spectroelectrochemistry for the Study of Microbial Extracellular Electron Transfer

Cited by 14 publications

References 31 publications

Long-Term Behavior of Defined Mixed Cultures of Geobacter sulfurreducens and Shewanella oneidensis in Bioelectrochemical Systems

Long-Term Behavior of Defined Mixed Cultures of Geobacter sulfurreducens and Shewanella oneidensis in Bioelectrochemical Systems

Copper-bottomed: electrochemically active bacteria exploit conductive sulphide networks for enhanced electrogeneity

In Situ and Operando Techniques for Investigating Electron Transfer in Biological Systems

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