In-Situ Biocatalytic Production of Trehalose with Autoinduction Expression of Trehalose Synthase

Abstract

We developed an in-situ biocatalytic process that couples autoinduction expression of trehalose synthase (TreS) and whole-cell catalysis for trehalose production. With lactose as the autoinducer, the activity of recombinant TreS in recombinant Escherichia coli was optimized through a visualization method, which resulted in a maximum value of 12 033 ± 730 U/mL in pH-stat fed-batch fermentation mode. Meanwhile, the permeability of the autoinduced E. coli increased significantly, which makes it possible to be directly used as a whole-cell biocatalyst for trehalose production, whereby the byproduct glucose can also act as an extra carbon source. In this case, the final yield of trehalose was improved to 90.5 ± 5.7% and remained as high as 83.2 ± 5.0% at the 10th batch, which is the highest value achieved using recombinant TreS. Finally, an integrated strategy for trehalose production was established, and its advantages compared to the traditional mode have been summarized.

Improved Expression and Optimization of Trehalose Synthase by Regulation of Pglv in Bacillus subtilis

“…Lactose, as an inducer instead of IPTG, is more economical and easier to obtain 28 . Furthermore, lactose can be completely consumed as a carbon source during the fermentation process, leaving no residue or product in the medium 29 . The recombinant B .…”

Permeabilized TreS-Expressing Bacillus subtilis Cells Decorated with Glucose Isomerase and a Shell of ZIF-8 as a Reusable Biocatalyst for the Coproduction of Trehalose and Fructose

“…In addition, a major impediment to using whole cells for trehalose production is the fact that TreS is an intracellular enzyme so that the cell wall and cell membrane would hinder the transfer of the substrate and the product. In our previous studies, it was verified that permeabilization treatment could markedly increase the yield of trehalose, but permeabilized cells tend to be more fragile, with dramatically decreased performance after a few rounds of recycling and changes of environmental factors. It was reported that coating a layer of MOFs on the cell surface can largely protect them from the influence of the external environment, making them resistant to radiation, high temperatures, and increased salt concentrations, thus preventing biological, thermal, and chemical degradation. − Studies have shown that the native cell wall can attract metal ions and enzymes.…”

“…Our team achieved a maltose conversion rate of more than 90% using permeabilized E. coli as a whole-cell biocatalyst . However, the production of trehalose using E.…”

Trehalose: As Sweet as Effective in Biomedical Research and Biotechnology

“…On the other hand, some groups are developing methodologies to introduce trehalose in different areas, as cosmetics (development of bath oils, moisturizers, due to its role as protection against dehydration); pharmaceutical applications (as component in medicines to treat high blood pressure, due to its role to protects against osmotic stress) [14]. Trehalose can be found in some methods to produce and preserve dried vegetables and fruits, in the production of Swiss cheeses and as sweetener [15,16]. One of the problems faced during therapeutic treatment, which use drugs that need to be delivered inside the organism, is how to introduce this component without side effects to the organism and with high rates of absorption.…”