In Situ Cartilage Regeneration with Acellular Bone Matrix in a Preclinical Model

Dai, Linghui; He, Zhenming; Jiang, Yanfang; Zhang, Xin; Ren, Shuang; Zhu, Jingxian; Shao, Zhenxing; Huang, Hongjie; Zhang, Jiying; Fu, Xin; Duan, Xiaoning; Hu, Xiaoqing; Ao, Yingfang

doi:10.2139/ssrn.3276400

Cited by 1 publication

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“…It has been reported that allogeneic ADM can result in neo-cartilage formation similar to native cartilage [ 16 , 38 ]. It was thought that ADM is highly accessible and easy to manufacture, with wide resources and low costs, compared to other acellular materials, such as ACM and acellular bone matrix (ABM) [ 8 , 17 , 39 ]. The use of allograft or exogenous cells in this research might have limited their clinical applications in the future.…”

Section: Discussionmentioning

confidence: 99%

“…This method could achieve the one-step repair of an articular cartilage injury without secondary surgery or the use of endogenous BMSCs and without donor site morbidity. Preliminary studies have confirmed that this method is feasible and has excellent effects for cartilage repair [ 6 , 7 , 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…To resolve these difficulties, a promising method has recently emerged to achieve one-step cartilage repair using tissue engineering techniques. This involves the combination of the appropriate biomaterials with endogenous BMSCs [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Using a Xenogeneic Acellular Dermal Matrix Membrane to Enhance the Reparability of Bone Marrow Mesenchymal Stem Cells for Cartilage Injury

Shi

Meng

et al. 2023

Bioengineering

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Due to its avascular organization and low mitotic ability, articular cartilage possesses limited intrinsic regenerative capabilities. The aim of this study is to achieve one-step cartilage repair in situ via combining bone marrow stem cells (BMSCs) with a xenogeneic Acellular dermal matrix (ADM) membrane. The ADM membranes were harvested from Sprague-Dawley (SD) rats through standard decellularization procedures. The characterization of the scaffolds was measured, including the morphology and physical properties of the ADM membrane. The in vitro experiments included the cell distribution, chondrogenic matrix quantification, and viability evaluation of the scaffolds. Adult male New Zealand white rabbits were used for the in vivo evaluation. Isolated microfracture was performed in the control (MF group) in the left knee and the tested ADM group was included as an experimental group when an ADM scaffold was implanted through matching with the defect after microfracture in the right knee. At 6, 12, and 24 weeks post-surgery, the rabbits were sacrificed for further research. The ADM could adsorb water and had excellent porosity. The bone marrow stem cells (BMSCs) grew well when seeded on the ADM scaffold, demonstrating a characteristic spindle-shaped morphology. The ADM group exhibited an excellent proliferative capacity as well as the cartilaginous matrix and collagen production of the BMSCs. In the rabbit model, the ADM group showed earlier filling, more hyaline-like neo-tissue formation, and better interfacial integration between the defects and normal cartilage compared with the microfracture (MF) group at 6, 12, and 24 weeks post-surgery. In addition, neither intra-articular inflammation nor a rejection reaction was observed after the implantation of the ADM scaffold. This study provides a promising biomaterial-based strategy for cartilage repair and is worth further investigation in large animal models.

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