In situ demineralisation of human enamel studied by synchrotron-based X-ray microtomography – A descriptive pilot-study

Lautensack, Julia; Rack, Alexander; Redenbach, Claudia; Zabler, Simon; Fischer, Horst; Gräber, Hans-Georg

doi:10.1016/j.micron.2012.09.006

Cited by 9 publications

(7 citation statements)

References 34 publications

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“…Multiplying the value of * by the average density of sound enamel will yield an approximate measure for the average mineral density within a given voxel. A similar normalization scheme for characterizing demineralization in human enamel has been previously reported by Lautensack and colleagues (Lautensack et al, 2013). 2.3.2.…”

Section: Figurementioning

confidence: 73%

Characterization of enamel caries lesions in rat molars using synchrotron X-ray microtomography

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DeRocher

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et al. 2017

J Synchrotron Radiat

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Dental caries is a ubiquitous infectious disease with a nearly 100% lifetime prevalence. Rodent caries models are widely used to investigate the etiology, progression and potential prevention or treatment of the disease. To explore the suitability of these models for deeper investigations of intact surface zones during enamel caries, the structures of early-stage carious lesions in rats were characterized and compared with previous reports on white spot enamel lesions in humans. Synchrotron X-ray microcomputed tomography non-destructively mapped demineralization in carious rat molar specimens across a range of caries severity, identifying 52 lesions across the 30 teeth imaged. Of these lesions, 13 were shown to have intact surface zones. Depth profiles of fractional mineral density were qualitatively similar to lesions in human teeth. However, the thickness of the surface zone in the rat model ranges from 10 to 58 µm, and is therefore significantly thinner than in human enamel. These results indicate that a fraction of lesions in rat caries possess an intact surface zone and are qualitatively similar to human lesions at the micrometer scale. This suggests that rat caries models may be a suitable analog through which to investigate the structure of surface zone enamel and its role during dental caries.

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Section: Figurementioning

confidence: 73%

Characterization of enamel caries lesions in rat molars using synchrotron X-ray microtomography

Free

DeRocher

Stock

et al. 2017

J Synchrotron Radiat

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“…Such differences are even found for in vitro tests with artificial lesions and are manifested by inter-lab variability [41,42], implying that the challenging biomimetic in vitro generation of carious lesions. Thus, in situ reproduction of lesions was proposed [43]. Furthermore, the classically described lesion is by no means representative of all observed natural lesions [44].…”

Section: Discussionmentioning

confidence: 99%

Automated Analysis of Spatially Resolved X-ray Scattering and Micro Computed Tomography of Artificial and Natural Enamel Carious Lesions

et al. 2018

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Radiography has long been the standard approach to characterize carious lesions. Spatially resolved X-ray diffraction, specifically small-angle X-ray scattering (SAXS), has recently been applied to caries research. The aims of this combined SAXS and micro computed tomography (µCT) study were to locally characterize and compare the micro-and nanostructures of one natural carious lesion and of one artificially induced enamel lesion; and demonstrate the feasibility of an automated approach to combined SAXS and µCT data in segmenting affected and unaffected enamel. Enamel, demineralized by natural or artificial caries, exhibits a significantly reduced X-ray attenuation compared to sound enamel and gives rise to a drastically increased small-angle scattering signal associated with the presence of nanometer-size pores. In addition, X-ray scattering allows the assessment of the overall orientation and the degree of anisotropy of the nanostructures present. Subsequent to the characterization with µCT, specimens were analyzed using synchrotron radiation-based SAXS in transmission raster mode. The bivariate histogram plot of the projected data combined the local scattering signal intensity with the related X-ray attenuation from µCT measurements. These histograms permitted the segmentation of anatomical features, including the lesions, with micrometer precision. The natural and artificial lesions showed comparable features, but they also exhibited size and shape differences. The clear identification of the affected regions and the characterization of their nanostructure allow the artificially induced lesions to be verified against selected natural carious lesions, offering the potential to optimize artificial demineralization protocols. Analysis of joint SAXS and µCT histograms objectively segmented sound and affected enamel.

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“…Lautensack et al (23) reported that synchrotron X-ray μCT yielded high-resolution images and was able to show variations in density inside the enamel structure. They concluded that quantitative image analysis methods allow for visual examination and quantification of the degree of demineralization in different samples.…”

Section: Discussionmentioning

confidence: 99%

Micro-computed tomographic analysis of progression of artificial enamel lesions in primary and permanent teeth after resin infiltration

2015

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In situ demineralisation of human enamel studied by synchrotron-based X-ray microtomography – A descriptive pilot-study

Cited by 9 publications

References 34 publications

Characterization of enamel caries lesions in rat molars using synchrotron X-ray microtomography

Characterization of enamel caries lesions in rat molars using synchrotron X-ray microtomography

Automated Analysis of Spatially Resolved X-ray Scattering and Micro Computed Tomography of Artificial and Natural Enamel Carious Lesions

Micro-computed tomographic analysis of progression of artificial enamel lesions in primary and permanent teeth after resin infiltration

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