In situ effect of enamel salivary exposure time and type of intraoral appliance before an erosive challenge

Mendonça, Fernanda Lyrio; Jordão, Maísa Camillo; Ionta, Franciny Querobim; Buzalaf, Marília Afonso Rabelo; Honório, Heitor Marques; Wang, Linda

doi:10.1007/s00784-016-2043-5

Cited by 23 publications

(38 citation statements)

References 54 publications

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“…Thus, it is possible that for the AEP challenged with intrinsic acid (HCl), a long time of maturation is important for the protection against demineralization. In line with this hypothesis, an in situ study showed that exposure to saliva for 30 min for AEP formation promoted less enamel resistance to HCl erosive attack than exposure to saliva for 2 h [Mendonça et al, 2017]. This suggests that more mature pellicles might provide higher protection against intrinsic erosion than short-term pellicles.…”

Section: And Itsupporting

confidence: 54%

Changes in the Proteomic Profile of Acquired Enamel Pellicles as a Function of Their Time of Formation and Hydrochloric Acid Exposure

et al. 2018

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Objective: Changes in the protein profile of acquired enamel pellicles (AEP) formed in vivo over different time periods were evaluated after the application of hydrochloric acid (HCl). Methods: Nine subjects were submitted to dental prophylaxis with pumice. After 3 or 120 min, the teeth were isolated with cotton rolls and 50 μL of 0.1 M HCl (pH 1.0), 0.01 M HCl (pH 2.0), or deionized water were applied on the buccal surface of the teeth for 10 s. The AEP was then collected using an electrode filter paper presoaked in 3% citric acid. After protein extraction, the samples were submitted to reverse-phase liquid chromatography coupled to mass spectrometry (nano LC-ESI-MS/MS). Label-free quantification was performed (Protein Lynx Global Service software). Results: A total of 180 proteins were successfully identified in the AEP samples. The number of identified proteins increased with the time of pellicle formation. Only 4 proteins were present in all the groups (isoforms of IgA, serum albumin, and statherin). The greatest number of proteins identified uniquely in one of the groups was obtained for the groups treated with HCl after 2 h of pellicle formation (approx. 50 proteins). Conclusion: Proteins resistant to removal by HCl, such as serum albumin and statherin, were identified even in the short-term AEP. In addition, 120-min pellicles present many proteins that are resistant to removal by HCl. This suggests an increase in protection against intrinsic acids with the time of pellicle formation, which should be evaluated in future studies.

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Section: And Itsupporting

confidence: 54%

Changes in the Proteomic Profile of Acquired Enamel Pellicles as a Function of Their Time of Formation and Hydrochloric Acid Exposure

et al. 2018

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“…Initial erosion lesions were induced by hydrochloric acid exposure, which has been used in several in situ studies [9,23,25,26] to simulate the acidity of the gastric juice, often an intrinsic cause for the development of dental erosion [27]. The erosive challenge was performed ex vivo due to ethical restrictions and to guarantee greater control and standardization of the demineralization.…”

Section: Discussionmentioning

confidence: 99%

“…The intraoral palatal appliances were made with acrylic resin on a plaster model of the upper arch of each volunteer; a new appliance was made for each phase of the study [21,23]. Acrylic appliances had two vertical rows, one on the right and the other on the left side, with one cavity (12 × 6 × 3 mm) for the fixation of two blocks.…”

Section: Volunteers and In Situ Experimentsmentioning

confidence: 99%

“…Blocks were fixed with wax and carefully leveled to the appliance surface to avoid dental plaque accumulation. An orthodontic wire was fixed with resin above the enamel blocks but without touching their surfaces to avoid tongue contact [22,23].…”

Section: Volunteers and In Situ Experimentsmentioning

confidence: 99%

“…In each phase, volunteers brushed their teeth with fluoride toothpaste 1 h prior to insertion of the intraoral appliances (8 am), aiming to standardize the amount of residual fluoride present in the month. They were instructed to wear the appliances for 2 hours to allow the formation of acquired pellicle [23].…”

Section: Volunteers and In Situ Experimentsmentioning

confidence: 99%

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In Situ Effect of Intra-Oral Application of Pastes Containing CPP-ACP or CPP-ACPF Against Initial Enamel Erosion

Fernandes

Alencar

Melo

et al. 2019

Pesqui. bras. odontopediatria clín. integr.

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Objective: To evaluate in situ the effect of toothpastes containing casein phosphopeptideamorphous calcium phosphate (CPP-ACP) and casein phosphopeptide-amorphous calcium phosphate associated to fluoride (CPP-ACPF) on initial erosion prevention. Material and Methods: Bovine enamel blocks (n = 192) were randomly assigned into 4 phases according to the baseline surface hardness: GI: CPP-ACP Paste (MI Paste ™), GII: CPP-ACPF Paste (MI Paste Plus ™), GIII: Fluoridated paste and GIV: Placebo Paste. In each of the 4 crossover phases, twelve volunteers wore intraoral palatal appliances containing 4 enamel blocks for 2 hours, then the tested treatments were applied intraorally (3 min) and the appliance was maintained in the mouth for another 3 hours. After, the appliances were removed and immersed in hydrochloric acid (0.01 M, pH 2.3) for 30 seconds to promote erosive demineralization. The final surface hardness was evaluated and percentage of surface hardness loss was calculated. The data were analyzed by ANOVA and Tukey's test (α = 5%). Results: The application of CPP-ACP paste, independent of fluoride content, resulted in significant lower enamel hardness loss (GI: 9.26% ±3.48 and GII: 9.14% ±1.73) compared to NaF (GIII: 15.5% ± 3.94) and placebo (GIV: 16.7% ± 4.07) pastes, which did not show difference between them. Conclusion: The CPP-ACP pastes were able to reduce initial erosive demineralization in relation to fluoride and placebo pastes. Nevertheless the formulation of CPP-ACP with fluoride did not provide an additional benefit.

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Is the dentifrice containing calcium silicate, sodium phosphate, and fluoride able to protect enamel against chemical mechanical wear? An in situ/ex vivo study

et al. 2019

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In situ effect of enamel salivary exposure time and type of intraoral appliance before an erosive challenge

Abstract: This finding will help researchers in the development of erosion studies, which will provide information for dentists to offer a better treatment for erosion.

Cited by 23 publications

References 54 publications

Changes in the Proteomic Profile of Acquired Enamel Pellicles as a Function of Their Time of Formation and Hydrochloric Acid Exposure

Changes in the Proteomic Profile of Acquired Enamel Pellicles as a Function of Their Time of Formation and Hydrochloric Acid Exposure

In Situ Effect of Intra-Oral Application of Pastes Containing CPP-ACP or CPP-ACPF Against Initial Enamel Erosion

Is the dentifrice containing calcium silicate, sodium phosphate, and fluoride able to protect enamel against chemical mechanical wear? An in situ/ex vivo study

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