2021
DOI: 10.1038/s41598-021-94620-8
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In situ electroporation of mammalian cells through SiO2 thin film capacitive microelectrodes

Abstract: Electroporation is a widely used non-viral technique for the delivery of molecules, including nucleic acids, into cells. Recently, electronic microsystems that miniaturize the electroporation machinery have been developed as a new tool for genetic manipulation of cells in vitro, by integrating metal microelectrodes in the culture substrate and enabling electroporation in-situ. We report that non-faradic SiO2 thin film-insulated microelectrodes can be used for reliable and spatially selective in-situ electropor… Show more

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Cited by 6 publications
(3 citation statements)
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“…The second significant drawback is the high cellular mortality during the transfection procedure. However, as reported by O’Brien and Lummis, the use of lower pulse pressure (about 345 kPa) significantly reduces the degree of cell damage [ 48 ]. The gene gun delivery method continues to evolve.…”
Section: Methods Of Delivery Of Dna- and Rna-based Expression Vector...mentioning
confidence: 98%
“…The second significant drawback is the high cellular mortality during the transfection procedure. However, as reported by O’Brien and Lummis, the use of lower pulse pressure (about 345 kPa) significantly reduces the degree of cell damage [ 48 ]. The gene gun delivery method continues to evolve.…”
Section: Methods Of Delivery Of Dna- and Rna-based Expression Vector...mentioning
confidence: 98%
“…The position of the stimulation electric field on the recording electrode and the optimal distance for its placement are both intriguing topics in the study of the spread of electrical signals. Earlier research has explored the behavior of electrodes in various environments and cells, and Peter Fromherz and his colleagues [ 51 , 52 ] have developed a particularly convenient form of electrode that is now utilized for the extracellular recording of potentials on multielectrode arrays. There exist theoretical studies on the extracellular activity of brain neurons under current stimulation, but at present, there is no successful physical prototype of such a system, leaving many questions open for further investigation.…”
Section: Methodsmentioning
confidence: 99%
“…It requires clinicians to use laser-related physics knowledge flexibly based on mastering solid oral professional knowledge and using the laser correctly, otherwise, there will be abuse or even counterproductive effects. Lasers are still being updated and developed continuously, and doctors in various dental professions are trying to implement the use of lasers [11,12].…”
Section: Introduction 1laser Chemicalmentioning
confidence: 99%