In Situ Entrapment of Catalase within Macroporous Cryogel Matrix for Ethanol Oxidation: Flow-through Mode versus Batch Reactor

Akbayeva, Dina N.; Smagulova, Indira A.; Maksotova, Kuralay S.; Bakirova, Botagoz S.; Tatykhanova, Gulnur S.; Kudaibergenov, Sarkyt E.

doi:10.3390/catal13071075

Cited by 3 publications

(2 citation statements)

References 53 publications

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“…The protocol followed to synthesize salsolinol was an adaptation of Akbayeva et al, 2023 ( 56 ) to obtain a catalase-mediated oxidation of alcohol into acetaldehyde and the production of salsolinol in presence of DA via Pictet-Spenlger reaction. The blank consisted of bovine catalase (Sigma Aldrich, Italy) at 0.33 mg/mL (666.67–1666.67 units/mL) and DA hydrochloride (Sigma Aldrich, CAS No.…”

Section: Methodsmentioning

confidence: 99%

Receptor and metabolic insights on the ability of caffeine to prevent alcohol-induced stimulation of mesolimbic dopamine transmission

Maccioni,

Bassareo,

Talani

et al. 2024

Preprint

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The consumption of alcohol and caffeine affects the lives of billions of individuals worldwide. Although recent evidence indicates that caffeine impairs the reinforcing properties of alcohol, a characterization of its effects on alcohol-stimulated mesolimbic dopamine (DA) function was lacking. Acting as the pro-drug of salsolinol, alcohol excites DA neurons in the posterior ventral tegmental area (pVTA) and increases DA release in the nucleus accumbens shell (AcbSh). Here we show that caffeine, via antagonistic activity on A2A adenosine receptors (A2AR), prevents alcohol-dependent activation of mesolimbic DA function as assessed, in-vivo, by brain microdialysis of AcbSh DA and, in-vitro, by electrophysiological recordings of pVTA DA neuronal firing. Accordingly, while the A1R antagonist DPCPX fails to prevent the effects of alcohol on DA function, both caffeine and the A2AR antagonist SCH 58261 prevent alcohol-dependent pVTA generation of salsolinol and increase in AcbSh DA in-vivo, as well as alcohol-dependent excitation of pVTA DA neurons in-vitro. However, caffeine also prevents direct salsolinol- and morphine-stimulated DA function, suggesting that it can exert these inhibitory effects also independently from affecting alcohol-induced salsolinol formation or bioavailability. Finally, untargeted metabolomics of the pVTA showcases that caffeine antagonizes alcohol-mediated effects on molecules (e.g. phosphatidylcholines, fatty amides, carnitines) involved in lipid signaling and energy metabolism, which could represent an additional salsolinol-independent mechanism of caffeine in impairing alcohol-mediated stimulation of mesolimbic DA transmission. In conclusion, the outcomes of this study strengthen the potential of caffeine, as well as of A2AR antagonists, for future development of preventive/therapeutic strategies for alcohol use disorder.

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Section: Methodsmentioning

confidence: 99%

Receptor and metabolic insights on the ability of caffeine to prevent alcohol-induced stimulation of mesolimbic dopamine transmission

Maccioni,

Bassareo,

Talani

et al. 2024

Preprint

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“…As is well known, their interconnected macroporous structure can confer super absorbance with high and tuneable mechanical properties in hydrophilic and hydrophobic formulations. Cryogels have received remarkable attention in water remediation as adsorbents [ 4 , 5 ], in heterogeneous catalysis [ 6 , 7 , 8 ], drug delivery [ 9 , 10 , 11 ], and tissue engineering [ 12 , 13 , 14 , 15 ]. Whatever the final destination, materials require a specific grade of mechanical stability that can compete with other properties.…”

Section: Introductionmentioning

confidence: 99%

How the Crosslinker Amount Influences the Final Properties of Hydroxyethyl Methacrylate Cryogels

Proietto Salanitri,

Luzzi,

Caretti

et al. 2024

Gels

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The investigation of the mechanical, thermal, and adsorption properties of hydroxyethyl methacrylate (HEMA) cryogels as a function of a reactant ratio is herein reported to better address materials for specific applications. To this aim, cryogels have been synthesized using different monomer/crosslinker (N,N′-methylene-bisacrylamide–MBAA) ratios. The study of SEM images made it possible to identify the trend in the material’s macroporosity. As would be expected, the average measured pore width decreased as the amount of MBAA increased while the number of pores grew. Swelling capacity ranges from 8.7 gW/ggel (grams of water per gram of gel) to 9.3 gW/ggel. These values are strictly connected with the pore’s size and distribution, revealing that the water uptake for the most crosslinked sample is inferior to other samples. The equilibrium-adsorption capacity (Qe) towards the methylene violet (MV) was also assessed, revealing no remarkable differences after 24 h of a batch test. As expected, thermogravimetric analysis (TGA) also showed no significant changes in stability that ranged from a maximum weight loss temperature (T Max) of 420 °C to 425 °C, which increased as a function of crosslinker content. Conversely, compression strength measurements showed a notable difference of about 50% in modulus (Ec), moving from the higher to the lower HEMA/MBAA ratio. These new comparative results indicate how slight variations in the reactant’s ratio can steadily improve the mechanical properties of the HEMA cryogel without affecting its adsorption efficiency. This can be helpful in the design of materials for water and energy purposes. Since swelling properties are needed in the case of biomedical applications, the HEMA/MBAA ratio should be tuned versus high values.

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Receptor and metabolic insights on the ability of caffeine to prevent alcohol-induced stimulation of mesolimbic dopamine transmission

Bassareo,

Maccioni,

Talani

et al. 2024

Transl Psychiatry

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The consumption of alcohol and caffeine affects the lives of billions of individuals worldwide. Although recent evidence indicates that caffeine impairs the reinforcing properties of alcohol, a characterization of its effects on alcohol-stimulated mesolimbic dopamine (DA) function was lacking. Acting as the pro-drug of salsolinol, alcohol excites DA neurons in the posterior ventral tegmental area (pVTA) and increases DA release in the nucleus accumbens shell (AcbSh). Here we show that caffeine, via antagonistic activity on A 2A adenosine receptors (A 2A R), prevents alcohol-dependent activation of mesolimbic DA function as assessed, in-vivo, by brain microdialysis of AcbSh DA and, in-vitro, by electrophysiological recordings of pVTA DA neuronal firing. Accordingly, while the A 1 R antagonist DPCPX fails to prevent the effects of alcohol on DA function, both caffeine and the A 2A R antagonist SCH 58261 prevent alcohol-dependent pVTA generation of salsolinol and increase in AcbSh DA in-vivo, as well as alcohol-dependent excitation of pVTA DA neurons in-vitro. However, caffeine also prevents direct salsolinol- and morphine-stimulated DA function, suggesting that it can exert these inhibitory effects also independently from affecting alcohol-induced salsolinol formation or bioavailability. Finally, untargeted metabolomics of the pVTA showcases that caffeine antagonizes alcohol-mediated effects on molecules (e.g. phosphatidylcholines, fatty amides, carnitines) involved in lipid signaling and energy metabolism, which could represent an additional salsolinol-independent mechanism of caffeine in impairing alcohol-mediated stimulation of mesolimbic DA transmission. In conclusion, the outcomes of this study strengthen the potential of caffeine, as well as of A 2A R antagonists, for future development of preventive/therapeutic strategies for alcohol use disorder.

show abstract

In Situ Entrapment of Catalase within Macroporous Cryogel Matrix for Ethanol Oxidation: Flow-through Mode versus Batch Reactor

Cited by 3 publications

References 53 publications

Receptor and metabolic insights on the ability of caffeine to prevent alcohol-induced stimulation of mesolimbic dopamine transmission

Receptor and metabolic insights on the ability of caffeine to prevent alcohol-induced stimulation of mesolimbic dopamine transmission

How the Crosslinker Amount Influences the Final Properties of Hydroxyethyl Methacrylate Cryogels

Receptor and metabolic insights on the ability of caffeine to prevent alcohol-induced stimulation of mesolimbic dopamine transmission

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