In situ evaluation of gemcitabine–DNA interaction using a DNA-electrochemical biosensor

Human Cytochrome P450 (CYP1A2)‐dsDNA Interaction in situ Evaluation Using a dsDNA‐electrochemical Biosensor

“…The dsDNA oxidation showed two small oxidation peaks, Figure B, corresponding to the purine bases residues oxidation in the polynucleotide chain: desoxyguanosine (dGuo), at E pa = + 0.92 V , , and desoxyadenosine (dAdo), at E pa = + 1.18 V . The small oxidation peaks of dGuo and dAdo were due to the difficulty of the electron transfer from the inside of the double‐stranded rigid DNA to the GCE surface.…”

“…The CYP1A2‐poly[G] and CYP1A2‐poly[A] interactions, to detect preferential interaction with purine bases Gua and Ade, were investigated. The electrochemical behaviour of CYP1A2, and of control dsDNA, poly[G] and poly[A] , was also investigated, Figure .…”

“…The CYP1A2‐dsDNA incubated solutions after 1 h, 2 h, and 5 h, showed a greater decrease of the dGuo oxidation peak, Figure , and after 5 h an increase of the dAdo oxidation peak, due to the CYP1A2 enzyme conformational changes and greater exposure to the GCE surface of the CYP1A2 electroactive amino acids oxidation, peak at E p3a =+ 1.18 V, Figure A, which occurred superimposed to the dAdo oxidation peak, at E pa = + 1.18 V, Figure B , .…”

Electrochemical DNA biosensor based on poly(2,6-pyridinedicarboxylic acid) modified glassy carbon electrode for the determination of anticancer drug gemcitabine

“…They investigated the changes in the reduction peak of [Fe(CN) 6 ] 3-/4-, which is a mediator for the detection of GEM [25]. In another study, Buoro et al investigated the interaction between GEM and DNA using a DNA-electrochemical biosensor [26]. No electrochemical process related to the oxidation or reduction of GEM was observed at the GCE in the potential range between -0.70 and +1.40 V (vs. Ag/AgCl) in various electrolytes, such as PBS and acetate buffer solution (ABS).…”

Antidiabetic Drug Metformin Oxidation andin situInteraction with dsDNA Using a dsDNA‐electrochemical Biosensor

“…The METÀ dsDNA interaction mechanisms research is also important due to the possibility to better understand, as well as detecting, perturbations of the DNA doublehelical structure and oxidative DNA damage. The DNAelectrochemical biosensor [29][30][31][32][33][34][35][36], consists of an electrochemical transducer with DNA immobilized on its surface capable of detecting specific drugÀ DNA binding process [37,38], through electrochemical transduction.…”