In situ expression of 15 kDa interferon alpha responsive gene in the developing tooth germ of the mouse lower first molar

Akhter, Merina; Kobayashi, Ieyoshi; Kiyoshima, Tamotsu; Nagata, Kazuhiro; Wada, Hiroko; Ookuma, Yukiko; Fujiwara, Hiroaki; Honda, Jyun Ya; Sakai, Hidetaka

doi:10.1007/s10735-010-9277-3

Cited by 7 publications

(3 citation statements)

References 26 publications

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“…This result is in agreement with our finding of a significant difference in Atpase6 mRNA expression at E12.0 in our previous study (Yamaza et al 2001a). The signal patterns of Atpase6 were very similar to those of these genes from the thickened epithelium to the bell stages of the tooth germ development (Akhter et al 2005(Akhter et al , 2010Wada et al 2002;Xie et al 2007Xie et al , 2009Yamaza et al 2001b). In situ signals for Atpase6 mRNA were found at the tip zone of the dental epithelium invaginating into the underlying mesenchyme in the cap stage of the tooth germ.…”

Section: Discussionmentioning

confidence: 68%

“…In situ hybridization using the antisense RNA probe was performed according to the protocol described in our previous studies (Akhter et al 2005(Akhter et al , 2010Honda et al 2008;Kobayashi et al 2006;Wada et al 2002;Xie et al 2007Xie et al , 2009Yamaza et al 2001aYamaza et al , 2001b. The embryos were fixed in 4% paraformaldehyde (PFA) in diethylpyrocarbonate (DEPC)-treated phosphate buffered saline (PBS, pH 7.4) for 12 h. Heads dissected from the fixed embryos were embedded in OCT compound (Sakura Finetechnical Co. Ltd, Tokyo, Japan).…”

Section: In Situ Hybridization For Atpase6 Mrnamentioning

confidence: 99%

“…In addition, 47 highly expressed positive clones were also obtained from the E12.0 mandible (Yamaza et al 2001a). Minute in situ expression patterns of the thymosin beta-4 (Tb4), 15-kDa interferon alpha responsive gene (Ifrg15), phosphoglycerate kinase 1 (Pgk1), heat shock protein (Hsc73, Hsj2 and Hsp86), nucleolin and Set-a genes were observed in the developing lower first molar tooth germs (Akhter et al 2005(Akhter et al , 2010Honda et al 2008;Wada et al 2002;Xie et al 2007Xie et al , 2009Yamaza et al 2001b). Furthermore, tooth germ development was disturbed by the treatment with nucleolin anti-sense sulfursubstituted oligodeoxynucleotide (AS-S-ODN) in the cultured E11.0 mandible (Xie et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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In situ expression of the mitochondrial ATPase6 gene in the developing tooth germ of the mouse lower first molar

et al. 2011

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We previously performed cDNA subtraction between the mouse mandibles on embryonic day 10.5 (E10.5) in the pre-initiation stage of the odontogenesis and E12.0 in the late initiation stage to identify genes expressed at its beginning. Adenosine triphosphate synthase subunit a (Atpase6) is one of the highly expressed genes in the E12.0 mandible including tooth germs. In situ hybridization was conducted using the mouse mandibular first molar from E10.5 to E18.0 to determine the precise expression patterns of Atpase6 mRNA in the developing tooth germ. Atpase6 mRNA was strongly expressed in the presumptive dental epithelium and the underlying mesenchyme at E10.5, and in the thickened dental epithelium at E12.0 and E13.0. Strong in situ signals were observed in the epithelium at E14.0, and in the enamel organ excluded the area of the primary enamel knot at E15.0. Atpase6 was strongly expressed in the inner enamel epithelium, the adjacent stratum intermedium, and the outer enamel epithelium in the cervical loops from E16.0 to E18.0. In addition, strong Atpase6 signals were coincidently demonstrated in various developing cranio-facial organs. These results suggest that Atpase6 participates in the high energy-utilizing functions of the cells related to the initiation and the development of the tooth germ as well as those of the other cranio-facial organs.

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Section: Discussionmentioning

confidence: 68%

Section: In Situ Hybridization For Atpase6 Mrnamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In situ expression of the mitochondrial ATPase6 gene in the developing tooth germ of the mouse lower first molar

et al. 2011

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Multiple functional involvement of Thymosin beta-4 in tooth germ development

Ookuma

Kiyoshima

Kobayashi

et al. 2012

Histochem Cell Biol

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Thymosin beta-4 (Tβ4) is known to be ubiquitously involved in the actin monomer sequestering on the cytoskeleton. Our previous study showed specific temporal and special in situ expression pattern of Tβ4 mRNA in dental epithelial and mesenchymal cells in the developing tooth germ of the mouse lower first molar. In this study, we examined the functional implications of Tβ4 in the developmental course of the mouse lower first molar. An inhibition assay using Tβ4 antisense sulfur-substituted oligodeoxynucleotide (AS S-ODN) in cultured embryonic day 11.0 (E11.0) mandibles showed a significant growth inhibition of the tooth germ. However, no growth arrest of the cultured E15.0 tooth germ was observed by using Tβ4 AS S-ODN. The Tβ4 knockdown led to significantly decreased expression levels of type II/III runt-related transcription factor 2 (Runx2) and nucleolin (Ncl) in the cultured E11.0 mandibles. Since our previous studies proved that the inhibition of type II/III Runx2 and Ncl translations resulted in the developmental arrest of the tooth germ in the cultured E11.0 mandible, Tβ4 appears to play roles in tooth germ development via the regulation of the type II/III Runx2 and Ncl expressions. Tβ4 knockdown also resulted in decreased secretion of matrix metalloproteinase (Mmp)-2, a reduced cell motility activity and upregulation of E-cadherin in dental epithelial mDE6 cells. These results suggest that Tβ4 plays multiple functional roles in odontogenic epithelial cells in the early stages of tooth germ development by regulating the expression of odontogenesis-related genes.

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Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) expression and possible function in mouse tooth germ development

et al. 2016

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Abnormal expression of Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) is involved in the pathogenesis of FSHD. FRG1 is also important for the normal muscular and vascular development. Our previous study showed that FRG1 is one of the highly expressed genes in the mandible on embryonic day 10.5 (E10.5) than on E12.0. In this study, we investigated the temporospatial expression pattern of FRG1 mRNA and protein during the development of the mouse lower first molar, and also evaluated the subcellular localization of the FRG1 protein in mouse dental epithelial (mDE6) cells. The FRG1 expression was identified in the dental epithelial and mesenchymal cells at the initiation and bud stages. It was detected in the inner enamel epithelium at the cap and early bell stages. At the late bell and root formation stages, these signals were detected in ameloblasts and odontoblasts during the formation of enamel and dentin matrices, respectively. The FRG1 protein was localized in the cytoplasm in the mouse tooth germ in vivo, while FRG1 was detected predominantly in the nucleus and faintly in the cytoplasm in mDE6 cells in vitro. In mDE6 cells treated with bone morphogenetic protein 4 (BMP4), the protein expression of FRG1 increased in cytoplasm, suggesting that FRG1 may translocate to the cytoplasm. These findings suggest that FRG1 is involved in the morphogenesis of the tooth germ, as well as in the formation of enamel and dentin matrices and that FRG1 may play a role in the odontogenesis in the mouse following BMP4 stimulation.

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In situ expression of 15 kDa interferon alpha responsive gene in the developing tooth germ of the mouse lower first molar

Cited by 7 publications

References 26 publications

In situ expression of the mitochondrial ATPase6 gene in the developing tooth germ of the mouse lower first molar

In situ expression of the mitochondrial ATPase6 gene in the developing tooth germ of the mouse lower first molar

Multiple functional involvement of Thymosin beta-4 in tooth germ development

Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) expression and possible function in mouse tooth germ development

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